对羟基苯甲酸处理对采后柑橘炭疽病的抑制及机理研究  

Mechanism and inhibitory effect of p-hydroxybenzoic acid on anthracnose in postharvest citrus

作　　者：黄小兰[1] 盖智星 王日葵[1,2,3] 贺明阳[1,2,3] 韩冷[1,2,3] 周炼[1,2,3] 

机构地区：[1]西南大学柑桔研究所,重庆400712 [2]中国农业科学院柑桔研究所,重庆400712 [3]国家柑桔工程技术研究中心,重庆400712

出　　处：《食品与机械》2016年第9期121-125,208,共6页Food and Machinery

基　　金：公益性行业(农业)科研专项经费项目(编号:201203034);重庆市科技支撑示范工程项目(编号:cstc2014fazktjcsf80007);中央高校基本科研业务费专项基金项目(编号:XDJK2013C014)

摘　　要：采用体外平板接菌试验和体内果实接种试验测定对羟基苯甲酸对采后柑橘炭疽病致病菌(胶孢炭疽菌)的生长抑制作用;观测经对羟基苯甲酸处理后,接种和未接种病原真菌的柑橘果实中多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)活性、丙二醛(MDA)含量的动态变化。结果表明:对羟基苯甲酸对采后柑橘炭疽病病原菌有较强的抑菌活性,浓度越高,对病原菌的抑制作用越强,其EC50为0.056mg/mL。经对羟基苯甲酸处理的柑橘果实中,PPO、POD、SOD、PAL活性升高,MDA含量下降。并且对羟基苯甲酸诱导的抗性相关酶在整个试验过程中维持较高的活性。对羟基苯甲酸处理对采后柑橘炭疽病病原菌有较强的抗菌作用,能提高柑橘果实相关抗性酶活性以及抑制膜脂过氧化反应,诱导其果实抗性。对柑橘采后贮藏保鲜具有重要意义。To explore the antifungal activity and potential mechanismof p-Hydroxybenzoic acid against Colletotrichum gloeosporioides in postharvest Citrus. The addition of p HBA in-vitro and in-vivo was conducted to test the inhibitory effect. Dynamic changes of peroxidase （POD）, polyphenoloxidase（PPO）, Superoxide Dismutase （SOD）, Ammonia-lyase （PAl.）activity and the content of Malondi- aldehyde（MDA） with different treatment was studied in postharvest citrus, p-HBA displayed strong antifungal activity to C. gloeospori- oides,when the concentration was higher, the effect was increased. The value of ECsois 0.056 mg/mL. The activities of PPO, POD, SOD and PAl. were increased when inoculated with p HBA and C. gloeosporioides, while the content of MDA was decreased, p- Hydroxybenzoie acid has a strong antimierobial activity, which can induce the increase of defending enzyme＇s activity and decrease the peroxidation of membrane lipid, The significance obviously is there for citrus fresh-keeping.

关 键 词：对羟基苯甲酸 胶孢炭疽菌 柑橘 贮藏保鲜 抑菌活性 抗氧化酶活性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]