油菜蜂花粉中粗黄酮提取物成分分析  

The study of rough flavones components extracted from the rape bee pollen

作　　者：王满生[1,2] 曾新安[2] 熊夏宇[2] 戴求仲[1,3] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205 [2]华南理工大学食品科学与工程学院,广东广州510640 [3]湖南省畜牧兽医研究所动物营养与饲养技术研究室,湖南长沙410131

出　　处：《食品与机械》2016年第9期144-148,共5页Food and Machinery

基　　金：国家自然科学基金项目(编号:21376094;21576099);广东省科技攻关项目(编号:2015A030312001;2013B020203001;2013B091100004)

摘　　要：为了明晰油菜蜂花粉中黄酮类物质的组成及脉冲电场辅助提取效果,试验采用TLC—DPPH和HPLC等方法对油菜蜂花粉的乙醇提取物及其酸解产物进行了详细的组分分析。结果表明,油菜蜂花粉的乙醇提取物中槲皮素和山奈酚因含量过低而未能检出,但该提取物经酸水解后,则会形成大量槲皮素和山奈酚,故可初步推断油菜蜂花粉中的黄酮类化合物主要以黄酮苷的形式存在。未经脉冲电场破壁处理的蜂花粉乙醇提取物的酸解物中,槲皮素含量为764.39μg/g,山奈酚含量为1 647.97μg/g,而经破壁处理后,槲皮素含量达867.93μg/g,对比提高了13.55%,山奈酚含量达1 924.03μg/g,对比提高了16.75%。该结果充分证明了脉冲电场预处理对油菜蜂花粉细胞具有良好的破壁效果。In order to clarify the flavonoids compositions of rape bee pollen and evaluate the assisted extraction effect for pulsed electric field （PEF）, the ethanol extract of rape bee pollen and its acidolysis component were analyzed in detail by the method of TLCDPPH and HPLC in this paper. The results showed that the quercetin and kaempferoI in the ethanol extract of rape bee pollen could not be de teeted for their low contents. However, a large number of quercetin and kaempferol were generated after the acidolysis of ethanolextract, and this implied that the flavonoids compositions of rape bee pollen mainly existed in the form of flavonoid glycoside. In addition, the content of quercetin and kaempferol in the acidolysis of ethanol extract were 764.39 μg/g and 1 647.97 μg/g respectively when the acidolysis materials were not treated by PEF. Nevertheless, when the acidolysis of ethanol exlract was pretreated by the PI-2F after- wards, the content of quercein and kaempferol reached 867.93 μg/g and 1 924.03 μg/g, increasing by 13.55% and 16.75% respectively. These results fully proved that the PEF technology pretreatment was an effective assisted extraction method and could be conducive to break the walls for the rape bee pollen cells.

关 键 词：油菜蜂花粉 黄酮类化合物 脉冲电场 薄层色谱 破壁 

分 类 号：TQ28[化学工程—有机化工]