肝素提取中膜分离回收固定化胰蛋白酶的工艺研究  

Research on preparation of heparin with immobilized enzyme combined with membrane technology

作　　者：于海宁[1] 张鹏[1] 沈生荣[2] 单伟光[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014 [2]浙江大学生物系统工程与食品科学学院,浙江杭州310058

出　　处：《浙江工业大学学报》2016年第5期515-518,共4页Journal of Zhejiang University of Technology

基　　金：浙江省科技厅重大科技专项资助项目(2014C03015)

摘　　要：以微滤膜分离肝素酶解液,回收固定化胰蛋白酶,研究蛋白酶重复提取肝素,具有较大的经济价值.通过单因素试验和正交试验,研究膜组件运行参数对膜分离回收固定化酶工艺的影响,通过考察肝素透过率、固定化胰蛋白酶拦截率、平均膜通量和膜通量恢复率为指标,获得膜分离回收固定化胰蛋白酶的最佳工艺条件和清洗陶瓷膜的最佳NaOH浓度.实验结果表明:在最佳膜分离条件下,固定化酶和肝素能够有效分离,平均膜通量达到656.13L/(m2·h),在该条件下回收的固定化胰蛋白酶仍具有良好的活性,可重复利用.采用最佳的2%NaOH溶液清洗陶瓷膜,陶瓷膜平均通量恢复至初始通量的97.9%.Using microfiltration membrane to separate heparin enzymolysis liquid and retrieve immobilized trypsin has a great economic value. Through single factor experiments and orthogonal experiments,heparin transmittance,trypsin interception rate,average volume flow of solution through membrane and membrane flux recovery rate were used as indicators to investigate the optimal concentration of NaOH and the optimal conditions of membrane separation.The result showed that under the optimal conditions,heparin and trypsin can be separated effectively.The average volume flow of solution through membrane was 656.13L/（m2·h）.Using 2%NaOH,the membrane could recover about 97.9% of the initial flux.

关 键 词：肝素 固定化酶 陶瓷膜 

分 类 号：R931.6[医药卫生—生药学]