北京市29例新确证HIV-1感染者多区段序列病毒亚型分析  

Sequence and subtype analysis of HIV-1 strains in gag,env and pol C2-V3 regions isolated from 29 newly-confirmed HIV-1 infectors in Beijing

作　　者：宋川[1] 陈丹瑛[1] 杜鹏程[1] 韩俊燕[1] 张媛媛[1] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所,新发突发传染病研究北京市重点实验室,北京100015

出　　处：《实用预防医学》2016年第11期1315-1318,共4页Practical Preventive Medicine

基　　金：北京市医院管理局“青苗”人才计划(QML20151701)

摘　　要：目的了解北京市男男性行为人群(MSM)中,29例新确证HIV-1感染者病毒株的基因序列特征。方法对HIV-1感染者进行流行病学调查,采集全血,分离血浆提取病毒核酸,应用套式反转录聚合酶链式反应(nested reverse transcription-polymerase chain reaction,nested RT-PCR)分别扩增gag、env和pol基因的部分片段,并对扩增产物进行测序,用Mega 5.0软件将测序结果与各亚型国际参考株比对,根据基因序列离散度确定系统进化树,综合三个区域的分型结果分析基因型及其特征。结果分析样本中扩增阳性的基因序列,结果表明至少存在5种不同的HIV-1亚型和重组模式,包括3种常见CRF01_AE(48.4%),CRF07/08_BC(24.1%),B/B'亚型(10.3%),以及罕见重组型CRF01_AE/CRF07_BC(6.9%)和CRF01_AE/B(10.3%);V3环顶端四肽分析发现,共有五种类型的顶端四肽:GPGQ、GPGR、GPGG、GPGL和GPGP,主要以GPGQ为主(73.3%);辅助受体分析结果发现,CRF01_AE亚型和CRF01_AE/B亚型的样本中出现了预测为X4嗜性的毒株。结论北京地区MSM人群中29例HIV-1感染者病毒株遗传多样性高,来源复杂,且存在罕见CRF01_AE/CRF07_BC和CRF01_AE/B重组型,应当继续加强HIV-1分子流行病学特征的监测。Objective To investigate the gene sequence characteristics of human immunodeficiency virus type-1 （HIV-1） strains derived from 29 newly confirmed HIV-1 infectors among men who have sex with men （MSM） in Beijing City. Methods An epidemiological survey was conducted in the 29 HIV-1 infectors and anticoagulating blood samples were collected. The plasma samples were isolated, and then RNA was extracted from each sample. The gag, env and pol genes were amplified by nested reverse transcription-polymerase chain reaction （nested RT-PCR） and the amplified products were sequenced. Phylogenetic analysis was performed based on genetic distance using Mega 5.0 software. The genetic subtypes were determined based on the constructed phylogenic tree. Results In this study, 15 of 29 samples were PCR positive within at least 2 targeted regions. Phylogenetic analysis revealed at least 5 variants of HIV-1 and recombinants among the HIV-1 infected MSM in Beijing City, including CRF01_AE （48.4%）, CRF07/08_BC （24.1%） and subtype B/B′ （10.3%） and 2 rarely reported recombinants, CRF01_AE/CRF07_BC （6.9%） and CRF01_AE/B （10.3%）. 5 types of 4 peptides motifs of V3-loop were found, including GPGQ, GPGR, GPGG, GPGL and GPGP. GPGQ was the predominant type （73.3%）. Co-receptor analysis revealed that most strains might utilize CCR5 as co-receptor to entry cells. But X4-tropic strains also appeared in the samples of CRF01_AE and CRF01_AE/B. Conclusions The genome sequences of HIV-1 strains among MSM in Beijing show high genetic diversity and complex origins; moreover, there exist 2 rarely reported recombinants, CRF01_AE/CRF07_BC and CRF01_AE/B. It is necessary to continuously strengthen the monitoring of molecular epidemiological characteristics of HIV-1.

关 键 词：男男性行为人群 HIV-1 基因测序 系统进化树 亚型特征 

分 类 号：R512.91[医药卫生—内科学]