马尾藻褐藻多糖硫酸酯的分离纯化及结构研究  

Purification and structure of fucoidan from Sargasso weed Sargassum

作　　者：张海霞[1] 汪秋宽[1] 何云海[1] 宋悦凡[1] 丛瑶 刘舒[1] 

机构地区：[1]大连海洋大学食品科学与工程学院,国家海藻加工技术研发分中心,辽宁省水产品加工及综合利用重点开放实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2016年第5期559-562,共4页Journal of Dalian Ocean University

基　　金：海洋公益性行业科研专项(201405040);辽宁省科技厅项目(2014205008)

摘　　要：为研究马尾藻Sargassum褐藻多糖硫酸酯的组成及结构,以马尾藻褐藻多糖硫酸酯粗品为原料,利用DEAE-Sepharose Fast Flow弱阴离子交换层析对粗品进行分离纯化,采用高效液相色谱法(HPLC)对粗品(F)及纯化后的两个组分(F0、F1)进行了单糖组成分析,同时利用红外光谱法、核磁共振波谱法研究了F0及F1的多糖结构。结果表明:用高效液相色谱法对单糖衍生物的分离效果良好,F、F0、F1均含有甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、木糖、岩藻糖;红外光谱分析显示,F和F0的硫酸基主要连接于岩藻糖的C2和C3位置。本研究结果可为马尾藻褐藻多糖硫酸酯的生物活性研究奠定基础。The fucoidan in Sargasso weed Sargassum was purified by DEAE-Sepharose Fast Flow anion-exchange chromatography and the structure characteristics of the fucoidan fractions（ F0 and F1） in the crude fucoidan（ F） were analyzed by infrared spectrum（ IR）,high performance liquid chromatography（ HPLC） and nuclear magnetic resonance（ NMR） spectroscopy to evaluate the monosarccharide composition and structure of fucoidan from Sargasso weed. The HPLC revealed that monosaccharide composition of these fractions were comprised of mannose,glucuronic acid,galactose,and fucose,with a little amount of rhamnose,glucose and xylose. The IR revealed that the sulfate site of F and F0 were located at Fuc C2 and C3. The findings provide basic data for the biological activity studies of the fucoidan from Sargasso weed.

关 键 词：马尾藻 褐藻多糖硫酸酯 纯化 结构分析 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]