茉莉花叶多酚的抗氧化作用研究  

Anti-oxidation Effect of Polyphenols from Jasmine Leaf

作　　者：商渊婷[1] 周志鹏[1] 李原[1] 甘家惠 黄锁义[2,3] 黄上峰[2,3] 

机构地区：[1]右江民族医学院临床学院,广西百色533000 [2]右江民族医学院药学院,广西百色533000 [3]右江流域特色民族药研究广西高校重点实验室,广西百色533000

出　　处：《中国野生植物资源》2016年第5期32-34,共3页Chinese Wild Plant Resources

基　　金：国家自然科学基金资助项目(81160347);国家中医药管理局"十二五"中医药重点学科中药化学学科建设项目(国中医药人教发[2012]2号);广西重点学科药物化学学科建设项目(桂教科研[2013]16号);广西壮族自治区百色市科学研究与技术开发计划第一批项目(百科计1001013)

摘　　要：目的:测定茉莉花叶乙酸乙酯提取物中多酚的含量及其体外抗氧化能力,为充分利用茉莉花资源提供理论依据。方法:用乙酸乙酯作溶剂,在75℃水浴中超声提取茉莉花叶多酚30 min,通过紫外-可见分光光度法测定了茉莉花叶中多酚的含量及其提取物的清除自由基能力。结果:茉莉花叶乙酸乙酯提取物中含有较高多酚含量,其提取物有较强的清除自由基能力,当提取物稀释10倍时,其抗氧化能力表现为:邻苯三酚法(89.2%)>Fenton法(77.0%)。茉莉花叶乙酸乙酯提取物的抗氧化能力随提取物浓度的增大而增加,二者呈现一定的量效关系。结论:茉莉花叶多酚具有较强的抗氧化活性。Objective：polyphenol content of ethyl acetate extract of Jasmine leaf and antioxidant capacity in vitro were determined, to provide a theoretical basis for the full use of the resources of jasmine. Meth od ： Using ethyl acetate as the solvent in a water bath at 75 ℃ ultrasonic extraction jasmine leaf polyphenols 30min, by UV - visible spectrophotometry the free radical scavenging ability of jasmine leaf polyphenol content and extract. Results：The ethyl acetate extract of Jasmine leaves contain high content of polyphenols, the extract has a strong free radical scavenging capacity. When the extract was diluted 10 - fold, its antioxidant capacity were as follows： pyrogallol method （89.2%） 〉 Fenton France （77.0%）. The ethyl acetate extract antioxidant capacity of Jasmine leaves increases with increasing extract concentration, showing a certain dose - effect relationship. Conclusion ： Jasmine leaf polyphenol has a strong antioxidant activity.

关 键 词：茉莉花叶 多酚 抗氧化性 

分 类 号：R284.2[医药卫生—中药学]