大鼠心肺复苏后脑Occludin蛋白表达与血脑屏障及脑水肿的关系  

Correlations of occludin protein expression with blood-brain barrier and cerebral edema in rats after cardiopulmonary resuscitation

作　　者：张慧利[1] 罗序睿[1] 李爱莲[1] 

机构地区：[1]南昌大学第一附属医院急诊科,330006

出　　处：《中国医药》2016年第11期1707-1712,共6页China Medicine

基　　金：江西省自然科学基金（20132BAB205015）

摘　　要：目的探讨大鼠心搏骤停心肺复苏后脑Occludin蛋白表达的变化及其与血脑屏障和脑水肿的关系。方法选取200只雄性SD大鼠，采用随机数字表法分为A组（对照组，100只，仅行麻醉、动静脉置管及气管插管）及B组（心肺复苏组，100只，制作心搏骤停与心肺复苏模型）；A组再分为A1、A2组，B组再分为B1、B2组，各50只。A1组于术后稳定10min后、B1组于自主循环恢复（ROSC）后2、6、12、24、48h各处死10只大鼠取脑组织，用免疫组化方法检测脑组织中Occludin蛋白表达，用干湿重法测脑组织含水量。A2组于术后稳定10min后、B2组于ROSC后即刻及4、10、22、46h各取10只大鼠于经尾静脉注射伊文思兰来示踪血脑屏障，注射后2h处死大鼠取脑组织，测定脑组织中伊文氏蓝含量。结果B，组ROSC后2、6、12、24、48h，脑Occludin蛋白阳性细胞数、脑组织含水量各时点之间差异均有统计学意义[Occludin蛋白阳性细胞数：（68．2±1．0）、（42．1±1．6）、（18.1±1．1）、（37．8±0．7）、（61．2±1．0）个／高位视野，脑组织含水量：（77．4±0．7）％、（79．2±0．8）％、（80．2±0．7）％、（79．6±0．5）％、（77．2±1．2）％]（P〈0．01），各时点与A．组[Occludin蛋白阳性细胞数：（86．8±1．3）、（85．1±2．7）、（84．9±2．8）、（82．1±3．1）、（83．8±2．5）个／高位视野，脑组织含水量：（76．0±0．9）％、（75．9±0．9）％、（76．2±1．0）％、（75．9±0．9）％、（75．8±0．9）％]比较差异均有统计学意义（P〈0．05或P〈0．01）；B，组脑组织伊文氏蓝含量各时点之间差异均有统计学意义[（0．50±0．04）、（0．70±0．02）、（0．90±0．04）、（0．87±0．03）、（0．69±0．03）μg／g]（P〈0．01），各时点与A2组[（0．24±0．01）、（0．23±0．01）、（0．23±0．01）、（0．25±0．01）、（0．23±0�Objective To explore the change of occludin protein expression after cardiopulmonary resuscitation（CPR） and its correlations with blood-brain barrier（BBB） and cerebral edema in rats. Methods Totally 200 male SD rats were randomly divided into group A （Sham group, n = 100, treated with anesthesa, arteriovenous intubation and tracheal intubation） and group B（ CPR group, n = 100, sudden cardiac arrest and CPR model） ; group A was subdivided into group A1 and group A2, group B were subdivided into group B1 and group B2, with respectively 50 rates in each subgroup. Group A1, B1 were used for testing occludin protein expression and cerebral water conten; rats were executed 2, 6, 12, 24, 48 h after 10 min of postoperative stability（group A1 ） or restoration of spontaneous circulation （ROSC） （group B 1 ）. Occludin protein expression was tested by immunohistochemical method; cerebral water content was tested by wet-dry weight method. Group A2 , B2 were used for evaluating the permeability of BBB; rats were injected Evans blue（EB） immediately and 4, 10, 22, 46 h after 10 min of postoperative stability（ group A2 ） or ROSC （group B2 ）, and they were executed 2 h after injection; cerebral EB content was tested. Results At 2, 6, 12, 24, 48 h after ROSC, occludin protein positive cell numbers and cerebral water contents in group B1 showed significant differences among each time point[ Occludin protein positive cell numbers ： （68.2 ± 1.0）, （42. 1 ± 1.6 ）, （ 18.1 ± 1.1 ）, （37. 8 ± 0. 7）, （61.2 ± 1.0）, cerebral water contents ： （77.4±0.7）%, （79.2 ±0.8）%, （80.2±0.7）%, （79.6 ±0.5）%, （77.2±1.2）%]（P〈0.01）, and there were significant differences between group A1 [ Occludin protein positive cell numbers ： （ 86. 8 ± 1.3 ） , （85.1 ± 2. 7 ）, （ 84. 9 ± 2. 8 ）, （ 82. 1 ± 3.1 ）, （ 83.8 ± 2. 5 ）, cerebral water contents： （76. 0 ± 0. 9 ） %, （75.9±0.9）%, （76.2±1.0）%, （75.9±0.9）%, （75.8±0.9）%1 a

关 键 词：心肺复苏 OCCLUDIN蛋白 血脑屏障 脑水肿 

分 类 号：R363.1[医药卫生—病理学]