Antioxidant and hepatoprotective activities of Dicoma anomala Sond.aqueous root extract against carbon tetrachloride-induced liver damage in Wistar rats  

作　　者：FO Balogun AOT Ashafa 

机构地区：[1]Phytomedicine and Phytopharmacology Research Group,Department of Plant Sciences,University of the Free State,Qwaqwa Campus

出　　处：《Journal of Traditional Chinese Medicine》2016年第4期504-513,共10页中医杂志（英文版）

基　　金：Supported by Research committee,University of the Free State,Qwaqwa campus,(entity No.211427604)Free State,SA

摘　　要：OBJECTIVE:To evaluates the antioxidant and hepatoprotective potentials of Dicoma anomala Sond.(Asteraceae) on body weight,feed and water intake,biochemical parameters and organ histology.METHODS:Various concentrations(1.56-25 μg/m L)were used in the in vitro assays 1,1-diphenyl-2-picryl hydrazyl(DPPH,superoxide anion,hydroxyl radicals,etc.).The effects of treatment with 125,250 and 250 mg/m L Dicoma anomala aqueous roots extract(DARE) was investigated in vivo in the CCl4-induced hepatotoxic rats during the 15 days study.RESULTS:Water extract exhibited the best activity(IC50:15.20 ± 0.03,11.70 ± 0.10,and 0.84 ± 0.05 μg/m L) in vitro in DPPH,hydroxyl and superoxide anion radicals,respectively,when compared with standards.Pre-treatment and treatment with different concentrations of DARE significantly(P < 0.05)attenuated the elevated serum activities of aspartate transaminase,alanine transaminase levels while increasing the activities of superoxide dismutase,catalase and glutathione peroxidase.The histopathological evaluations revealed extensive liver damage characterized by severe vacuolar and cytoplasmic degeneration,hepatic necrosis,and cellular infilteration in pre-treated groups while in the treated groups;such liver damages were not observed most especially at 500 mg/kg dose.CONCLUSION:The results proved the hepatoprotective potential of DARE against CCl4-induced oxidative stress.Moreover,histopathological examinations revealed better therapeutic advantage of DARE than prophylactic use.OBJECTIVE： To evaluates the antioxidant and hepa- toprotective potentials of Dicoma anomala Sond. （Asteraceae） on body weight, feed and water in- take, biochemical parameters and organ histology. METHODS： Various concentrations （1.56-25 μg/mL） were used in the in vitro assays 1,1-diphenyl-2-pic- ryl hydrazyl （DPPH, superoxide anion, hydroxyl radi- cals, etc.）. The effects of treatment with 125, 250 and 250 mg/mL Dicoma anomala aqueous roots ex- tract （DARE） was investigated in vivo in the CCI4-in- duced hepatotoxic rats during the 15 days study. RESULTS： Water extract exhibited the best activity （IC50：15.20 ± 0.03, 11.70 ± 0.10, and 0.84 ± 0.05 μg/ mL） in vitro in DPPH, hydroxyl and superoxide an- ion radicals, respectively, when compared with standards. Pre-treatment and treatment with differ- ent concentrations of DARE significantly （P 〈 0.05） attenuated the elevated serum activities of aspar-tate transaminase, alanine transaminase levels while increasing the activities of superoxide dis- mutase, catalase and glutathione peroxidase The histopathological evaluations revealed extensive liver damage characterized by severe vacuolar and cytoplasmic degeneration, hepatic necrosis, and cellular infilteration in pre-treated groups while in the treated groups; such liver damages were not observed most especially at 500 mg/kg dose.CONCLUSION： The results proved the hepatopro- tective potential of DARE against CCL-induced oxi- dative stress. Moreover, histopathological examina- tions revealed better therapeutic advantage of DARE than prophylactic use.

关 键 词：Dicoma anomala Sond (Asteraceae) Antioxidant enzymes Carbon tetrachloride HEPATOPROTECTION Lipid peroxidation 

分 类 号：R575[医药卫生—消化系统]