端基含乳糖的改性聚己内酯的酶法合成  

Lipase-catalyzed synthesis of terminal-functionalized poly(ε-caprolactone)with lactose

作　　者：戴亚平[1] 袁久刚[1] 范雪荣[1] 王强[1] 王平[1] 崔莉[1] 张颖[1] Dai Yaping Yuan Jiugang Fan Xuerong Wang Qiang Wang Ping Cui Li Zhang Ying(Key Laboratory of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 21412 2)

机构地区：[1]生态纺织教育部重点实验室,江南大学,无锡214122

出　　处：《化工新型材料》2016年第10期207-209,共3页New Chemical Materials

基　　金：国家自然科学基金资助项目(31300785)

摘　　要：为了改善聚己内酯(PCL)的细胞亲和性,在无溶剂体系中,以乳糖为引发剂,通过脂肪酶Novozyme-435催化ε-己内酯开环聚合反应,制备了端基含糖的改性PCL。红外光谱和核磁分析显示,乳糖成功接枝在PCL的末端。研究了反应温度、反应时间和水活度对己内酯的单体转化率的影响,以乳糖为引发剂时理想的反应条件为:反应温度60℃、时间24h、水活度0.57。引发剂的加入可以提高单体的初始转化率,但对最终转化率没有较大影响。Novozyme-435 lipase was used to catalyze the ring-opening polymerization of ε-caprolactone in solvent free system. Lactose was used as initiating agent for preparing the sugar end group modification of polycaprolactone to en- hance its cellular affinity. The FT-IR and 1 H-NMR results showed that the lactose was successfully grafted to carhoxyl ter- minal of poly（e-caprolactone）. The effects of temperature, reaction time, and water activity on monomer conversion were systematically evaluated. The results showed that the optimum reaction was reached at reaction temperature 60℃, time 24h, and water activity 0.57. The initial reaction rate increased with the addition of lactose, which had a negligible effect on the final conversion.

关 键 词：脂肪酶 Ε-己内酯 开环聚合 乳糖 

分 类 号：R318.08[医药卫生—生物医学工程]