苏云金芽孢杆菌酵解大西洋鲑加工废弃物制备抗氧化酵解液研究  

Study on the optimal conditions of preparing antioxidant hydrolysate from Atlantic salmon processing wastes using Bacillus Thuringiensis

作　　者：陈琳[1] 李秉钧[1] 冯俊荣[1] 于本淑 韩恭文 

机构地区：[1]烟台大学海洋学院,山东烟台264005 [2]山东省水生生物资源养护管理中心,山东烟台26400 [3]龙口市水产技术推广站,山东龙口265700

出　　处：《渔业现代化》2016年第5期28-35,共8页Fishery Modernization

基　　金：山东省科技发展计划项目(2012GHY11515)

摘　　要：为减少因大西洋鲑加工废弃物中的蛋白成分而造成的环境污染和资源浪费,同时提高其综合利用价值,探索以大西洋鲑加工废弃物制成的干粉为原料,使用一株苏云金芽孢杆菌,通过控制料液比、接种量、发酵时间、温度、pH 和菌龄等发酵条件来酵解鱼粉,以制取抗氧化酵解液.首先以总抗氧化活性和α-氨基氮含量为指标,找到最佳单因素条件;然后在单因素试验的基础上,以水解度和总抗氧化活性为指标,使用正交实验L25（56）对发酵条件进行优化,确定最佳发酵条件为：料液比2.0 g/50 mL,接种量3%,发酵时间72 h,温度30℃,pH 6.6,菌龄48 h,该条件下酵解得到的发酵液总抗氧化活性为503.59 U,鱼粉水解度为17.29%.研究表明,采用苏云金芽胞杆菌酵解大西洋鲑加工废弃物以制取抗氧化酵解液,具有一定的可行性.There are abundant proteins that are of high value in the processing residues of Atlantic salmon( Salmo salar); to avoid waste and environmental pollution caused by throwing them away,and to improve their comprehensive utilization value,the research was conducted where the dry powder made from Atlantic salmon processing residues were hydrolyzed by Bacillus Thuringiensis to produce antioxidant hydrolysate. The reaction conditions of the enzymatic hydrolysis including material- to- solvent ratio,inoculum quantity,time,temperature,pH and cell age were investigated. Firstly the best single factor was found by comparing the total antioxidant activity and amino nitrogen concentration; then based on single factor experiment and taking the degree of hydrolysis and the total antioxidant activity as indicators,orthogonal experimental design method was used to optimize the hydrolysis conditions,and the optimal conditions were determined by as follows: material-to- solvent ratio 0. 7 g /50 mL,inoculum quantity 3%,time 72 h,temperature 30 ℃,p H 6. 6 and cell age48 h; under such conditions the total antioxidant activity and degree of hydrolysis were 503. 59 U and 17.29%,respectively. In conclusion,it is feasible and practical to prepare antioxidant hydrolysate from Atlantic salmon processing wastes using Bacillus Thuringiensis.

关 键 词：大西洋鲑 废弃物 苏云金芽孢杆菌 酵解 抗氧化酵解液 正交实验 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]