Stathmin表达水平与乳腺癌细胞侵袭能力相关性研究  

Correlation of Breast Cancer Cells Invasive and Stathmin Gene Expression

作　　者：杨铭[1] 林芳[1] 和婷[1] 王琳[1] 董轲[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院临床实验与检验科

出　　处：《现代检验医学杂志》2016年第5期14-17,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金资助项目(81372836)

摘　　要：目的：探讨乳腺癌细胞 MDA-MB-231和 MCF-7中 Stathmin基因表达水平与细胞生长、黏附、侵袭等生物学行为之间的关系，为进一步研究乳腺癌转移机制奠定实验基础。方法应用 RT-PCR和 Western Blot方法检测 MDA-MB-231和 MCF-7细胞中 Stathmin基因的表达水平，同时利用细胞增殖试验、细胞黏附试验和细胞侵袭试验检测 MDA-MB-231和MCF-7细胞的生长、黏附、侵袭能力，分析Stathmin表达与细胞的生长、黏附、侵袭能力之间的关系。结果 RT-PCR和Western Blot检测结果显示，Stathmin基因在 MDA-MB-231和 MCF-7细胞中表达均高于正常对照细胞（F＝10.173，P＜0.05），且 MDA-MB-231细胞中的表达水平明显高于 MCF-7细胞中的表达水平（t＝4.562，P＜0.05）。而 MDA-MB-231细胞在生长、黏附和侵袭能力方面均强于 MCF-7细胞（P＜0.05）。结论 Stathmin表达水平高的乳腺癌细胞相应的生长、黏附、侵袭能力较强，Stathmin表达水平与细胞侵袭能力密切相关。Objective To explore the relationship between the Stathmin gene expression in breast cancer cells MDA-MB-231, MCF-7 and the biological behaviours such as cell growth,adhesion and invasion,and provide experimental basis of breast cancer metastasis for further study.Methods Used RT-PCR and Western Blot methods to detect the Stathmin gene expres-sion levels in MDA-MB-231 and MCF-7 cells,and in the mean while to test the MDA-MB-231 and MCF-7 cell growth,adhe-sion,invasion ability by CCK-8 cell proliferation experiments,cell adhesion experiments,cell invasion experiments,then, analyed the relationship of Stathmin gene expression and cell growth,adhesion,invasion ability.Results Over-expression levels of Stathmin gene were observed both in the MDA-MB-231 and MCF-7 cells （F=10.173,P〈0.05）,and furthermore, the expression levels of Stathmin gene in MDA-MB-231 cells was higher than in MCF-7 cells （t=4.562,P〈0.05）.While, the growth,adhesion and invasion ability of the MDA-MB-231 cells was higher than that of MCF-7 cells（P〈0.05）.Conclu-sion The higher level of Stathmin gene expression,the stronger breast cancer cells had ability of growth,invasion,and ad-hesive.The Stathmin gene expression levels was closely correlated with breast cancer cell invasive.

关 键 词：STATHMIN 乳腺癌 侵袭性 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]