影响乳酸菌生物膜形成的因素  

Study of the influence of factors for Lactobacillus biofilm formation

作　　者：张立冬[1] 党芳芳 赵思雨[1] 范颖[1] 王喆[1] 满朝新[2] 郭鸰[1] 姜毓君[1,2] 

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《中国乳品工业》2016年第10期4-6,14,共4页China Dairy Industry

基　　金：黑龙江省杰出青年基金项目(JC201415);黑龙江省院所创新能力提升专项计划项目(YC13D005)

摘　　要：对Tween 80、金属盐离子、碳源等因素对4株乳酸菌形成生物膜能力的影响进行了研究。结果显示:在MRS培养基中4株乳酸菌的生物膜形成能力的强弱表现为Lactobacillus reuteri>Lactobacillus plantarum NDC 75017>Lactobacillus paracasei>Lactobacillus rhamnosus GG。Tween 80和葡萄糖对其生物膜的形成均有抑制的作用,且葡萄糖的作用更为显著;Mn^(2+)则起到了一定的促进作用,而Mg^(2+)产生的影响由于菌株的不同而各异;葡萄糖为L.reuteri和L.plantarum生物膜形成的最佳碳源,而乳糖为L.paracasei和LGG生物膜形成的最佳碳源。The aim of this paper was to study the effects of the components, for example Tween 80 ,metal salt ions and carbon source to four different Lactobacillus＇ ability of biofilm fornation. The results showed that the ability of biofilm formation of the four lactobadllus were Lactoba. cillus, reuteri 〉 Lactobacillus. plantarum NDC 75017 〉 Lactobadllus. paracasei 〉 Lactobacillus. rhamnosus GG. The biofilm formation ability was re- stricted by omiited Tween 80 and glucose from MRS, and glucose was more significant for the capacity ofbiofilm formation; Mn2＋ could pro- mote the biofilm formation ability of the four Lactobacillus, but Mg2＋ didn＇t have aregulation because the different strains; glucose could seen as the best carbon source for the biofilm formation of L. reuteri and L. plantarum, lactose could seen as the best carbon source for the biofilm formation of L. paracasei and LGG.

关 键 词：生物膜 乳酸杆菌 培养基 成分 

分 类 号：Q93-33[生物学—微生物学]