链霉菌CSDX076次级代谢产物PseurotinA的分离及结构鉴定  

Isolation and structural identification of Pseurotin A from Streptomyces sp. CSDX076 secondary metabolite

作　　者：钱声艳 李宇真 邵美云[1] 程桂广[2] 保玉心[1] 岳昌武[1] 

机构地区：[1]遵义医学院医学与生物学研究中心,贵州省微生物资源及药物开发特色重点实验室,贵州遵义563000 [2]昆明理工大学化学工程学院,云南昆明650093

出　　处：《中国酿造》2016年第10期112-115,共4页China Brewing

基　　金：国家自然科学基金(31160004,31460006);贵州省科技学技术基金项目(黔科合J字[2010]2156,黔科合J字[2012]2348,黔科合SY字[2013]3013);遵义医学院硕士启动基金(F-757)

摘　　要：对从赤水丹霞山土壤样品中分离得到的链霉菌(Streptomyces sp.)CSDX076进行液体发酵。利用乙酸乙酯萃取,硅胶柱层析、薄层层析和反相中压柱层析对发酵液中的次级代谢产物进行分离、纯化;通过核磁共振法对纯化的分离产物进行结构鉴定。结果表明,该研究从链霉菌属中成功分离得到次级代谢产物Pseurotin A。采用滤纸片法对Pseurotin A进行抗菌活性测试,Pseurotin A对金黄色葡萄球菌具有抑制作用,其抑菌圈直径为5.0 mm。The Streptomyces sp. CSDX076 isolated from Chishui Danxia mountain soil samples was fermented by liquid culture medium. The secondary metabolite in the fermented liquid was separated and purified by ethyl acetate extraction, slica column chromatography, thin-layer chromatography and reverse phase medium pressure column chromatography. The structure of purified products was identified by nuclear magnetic resonance method. The results showed that the secondary metabolite Pseurotin A was separated and obtained from Streptomyces sp. successfully. The antimicrobial activity of Pseurotin A was determined by filtering paper method. The results showed that Pseurotin A had inhibiting effect on Staphylococcus aureus, and the diameter of antibacterial circle was 5.0 mm.

关 键 词：链霉菌CSDX076 次级代谢产物 Pseurotin A 结构鉴定 

分 类 号：Q935[生物学—微生物学]