糯玉米杂交种纯度InDel分子标记鉴定与田间鉴定的相关性分析  

The relativity between InDel method and field test in purity identification of waxy maize hybrids

作　　者：胡俏强[1] 陆海燕[2] 李炯 陈舜权[1] 吴月琴 戴惠学[1] HU Qiao-qiang LU Hai-yan LI Jiong CHEN Shun-quan WU Yue-qin DAI Hui-xue(Nanjing Institute of Vegetables Science ,Nanjing 210042, China Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China Shanghai Seed Industry （Group） Co., Ltd., Songjiang 201615, China)

机构地区：[1]南京市蔬菜科学研究所,江苏南京210042 [2]江苏省农业科学院农业生物技术研究所,江苏南京210014 [3]上海种业(集团)有限公司,上海松江201615

出　　处：《江苏农业学报》2016年第5期999-1004,共6页Jiangsu Journal of Agricultural Sciences

基　　金：上海市"科技创新行动计划"医学和农业领域科技支撑项目(14391900200);江苏省"六大人才高峰"第十二批高层次人才资助项目(2015-NY-036);南京市科技发展计划项目

摘　　要：为发展更快捷、简便的新型分子标记技术并研究其在糯玉米杂交种纯度鉴定中的应用,对4个糯玉米杂交种进行了InDel共显性分子标记纯度分析,筛选出5对多态性好的引物,并与田间鉴定结果进行了相关性分析。结果显示,InDel标记鉴定结果与田间鉴定结果呈极显著正相关,相关系数达到0.998。因此,InDel标记鉴定可用于糯玉米杂交种纯度鉴定,且结果准确可靠。To develop an efficient and simple molecular marker technique in the hybrid seed purity test of waxy maize, the purity of 4 waxy maize hybrids was analyzed by co-dominant InDel molecular markers. Five pairs of high poly- mophic primers were selected. Correlation analysis showed that the result of InDel markers identification was significantly positively correlated with that of field test, with the correlation coefficient of 0.998. InDel marker is a potential technique in hybrid seed purity identification of waxy maize.

关 键 词：糯玉米 INDEL 纯度鉴定 相关性 

分 类 号：S513.035.3[农业科学—作物学]