L-赖氨酸免疫磁性微球制备及其应用研究  

Research on the Separation of L-lysine Immunomagnetic Microspheres Serum Protein

作　　者：李鹤[1] 徐丹[1] 李晓凤[1] 

机构地区：[1]西华大学食品与生物工程学院,四川成都610039

出　　处：《食品研究与开发》2016年第15期135-138,共4页Food Research and Development

基　　金：四川省大学生创新创业训练计划项目(201510623055)

摘　　要：采用化学共沉淀法制备Fe_3O_4纳米微粒,并对其粒径大小、磁响应性和磁分散性进行综合分析。利用反向悬浮包埋法,以Fe_3O_4纳米微粒为载体制备L-赖氨酸高分子微球,并将其运用于猪血清中蛋白的分离。以猪血清蛋白偶联率为指标,通过正交试验优化L-赖氨酸高分子微球作用于猪血清蛋白的最佳作用条件。结果表明:最佳作用条件为反应温度40℃,作用时间3 h,血清用量20 m L,在此条件下测得猪血清蛋白的偶联率达到18.59%。This experiment used chemical Co-precipitable to get Fe3O4 particles, then analyzed its particle size, magnetic response and magnetic dispersion. Use Fe3O4 nanoparticles as carriers for the preparation of L-lysine polymer microsphere by reverse suspension method, which was applied to the separation of pig serum protein. Coupled rate with the pig＇s serum was used as a target to optimize the technical conditions through the orthogonal experiments. 40℃, the reaction time pling rate could reach to The results showed that the best condition was as follow, the reaction temperature was was 3 h and the serum dosage was 20 mL,under these conditions the serum protein coupling rate could reach to 18.59 %.

关 键 词：Fe_3O_4纳米微粒 L-赖氨酸 猪血清蛋白 偶联率 

分 类 号：O629.71[理学—有机化学]