人体胆结石难溶物的扫描电镜观察和红外光谱研究  

Study of the Insoluble Remainder of Human Gallstones by Scanning Electron Microscope and Fourier Transform Infrared Spectrum

作　　者：刘刚[1] 邢达[1] 杨海珉[2] 吴杰[2] 

机构地区：[1]华南师范大学激光生命科学研究所,广东广州510631 [2]昆明医学院基础部,云南昆明650031

出　　处：《光谱学与光谱分析》2002年第4期591-595,共5页Spectroscopy and Spectral Analysis

基　　金：国家杰出青年基金 (No 6972 50 0 9);广东省自然科学基金 (No 0 0 0 679)资助

摘　　要：用傅里叶变换红外光谱仪和扫描电镜研究了胆色素型和混合型胆结石经氯仿、乙醇、乙醚、盐酸等溶剂溶解剩余物的主要组分和微观结构。结果表明 ,胆红素盐、蛋白质、脂肪酸盐、磷酸盐、碳酸钙等为难溶物 ;棕色色素型胆石中的蛋白质的二级结构以α 螺旋为主。扫描电镜观察显示 ,在色素型和混合型胆石难溶物中都出现了可能为蛋白质的薄膜带状物 。The insoluble remainder of brown pigment and mixed gallstones which were.. dissolved with chloroform, ethanol, ether, and hydrochloric acid were studied by Fourier transform infrared (FT-IR) spectrum and scanning electron microscope. It was found that bilirubinate salts, proteins, fatty salts, phosphate and calcium carbonate are the main components in the insoluble remainder of gallstones. The secondary structure of proteins in brown pigment gallstones may be predominated by alpha-helix. The microstructure of proteins in brown pigment stones is membranous, phosphate and bilirubinate salts were trapped within the membranes. The. relationship between the insoluble components and formation of gallstones was discussed.

关 键 词：人体 胆结石 难溶物 扫描电镜 红外光谱 

分 类 号：R575.62[医药卫生—消化系统]