茶多酚对人前列腺癌PC-3M细胞中Survivin mRNA和蛋白表达的影响  

Effect of tea polyphenols on mRNA and protein expression of survivin in prostate cancer PC-3M cells

作　　者：严正强 杨远清 卢启海[2] 

机构地区：[1]宁波市医疗中心李惠利东部医院泌尿外科,315103 [2]广西科技大学附属柳州市人民医院泌尿外科

出　　处：《浙江医学》2016年第21期1727-1730,共4页Zhejiang Medical Journal

摘　　要：目的探讨茶多酚对人前列腺癌PC-3M细胞中调控细胞凋亡的生存素(Survivin)m RNA和蛋白表达的影响。方法在人前列腺癌PC-3M细胞培养器皿中分别加入用完全培养液稀释的0、40、60、80μg/ml茶多酚溶液,24、48h后应用RT-PCR和We s te rn b lot检测并比较Survivin m RNA、蛋白表达水平。结果与0μg/ml组相比,40μg/ml组加入茶多酚48h后Survivin m RNA、蛋白表达水平均明显下调(均P<0.05);60、80μg/ml组加入茶多酚24、48h后Survivin m RNA、蛋白表达水平均明显下调(均P<0.05),其中80μg/ml组下调最为明显(均P<0.05);大致呈剂量、时间依赖性(均P<0.05)。结论茶多酚可下调Survivin m RNA和蛋白表达水平,促进人前列腺癌PC-3M细胞调亡,在一定范围内呈剂量、时间依赖性。Objective To investigate the effect of tea polyphenols on the mRNA and protein expression of survivin in prostate cancer PC-3M cells. Methods Human prostate cancer PC-3M cells were treated with 40, 60, 80μg/ml tea polyphenol for 24h or 48h, the mRNA and protein expression of survivin in PC-3M cells were detected by RT-PCR and western blot,respectively. Results The mRNA and protein expression of survivin in PC-3M cells treated with 60μg/ml and 80μg/ml for 24h and 48h was significantly decreased ina dose-dependent and time-dependent manner（P〈0.05）. Conclusion Tea polyphenols can promote apoptosis of human prostate cancer PC-3M cells, which is related to the down-regulation of survivin gene tran-scription and translation.

关 键 词：茶多酚 前列腺癌 PC-3M细胞 细胞凋亡 生存素 

分 类 号：R737.25[医药卫生—肿瘤]