Real-timePCR在GB/T 4789.4-2010中的应用  

Application of Real-time PCR in GB/T 4789.4-2010

作　　者：刘万静 李湘平[1] 刘斌[1] 杜冬冬[1] 屈娅荣[1] 李海燕[1] 周惠萍[1] 

机构地区：[1]安康市疾病预防控制中心生物检验科,陕西安康725000

出　　处：《食品与发酵科技》2016年第5期68-70,共3页Food and Fermentation Science & Technology

摘　　要：通过应用Real-timePCR检测,提高沙门氏菌的检出率;同时简化实验程序,减少工作量,为可能发生的食物中毒尽早提供实验依据。选取2014、2015年国家食品风险监测标本。标准组按照GB/T 4789.4-2010进行沙门氏菌检测;实验组取GB/T 4789.4-2010中规定的第一次增菌液进行Real-timePCR,若阳性继续按照GB/T 4789.4-2010后续步骤检测,若阴性则直接报告结果。共检测370个标本,共检出沙门氏菌5株,标准组与实验组检测结果一致,符合率100%。通过应用Real-timePCR,可以为目的菌检出提供导向作用,有助于提高其检出率;对于PCRReal-timePCR阴性的标本,可提前3-4d直接报告,缩短了实验周期;由于后续只针对Real-timePCR阳性标本进行检测,减少了实验者的工作量,节约了阴性标本后续检测的实验试剂。To improve the detection rate of Salmonella by using Real-time PCR, and to simplify the experimental procedure and reduce the amount of work, so as to provide the experimental evidence for the possible occurrence of food poisoning. In 2014 and 2015, the national food risk monitoring samples were selected. The standard group for detection of Salmonella in accordance with the provisions of GB/T 4789.4-2010; the experimental group GB/T 4789.4-2010 for the first time in broth Real-time PCR, if the positive detection in GB/T 4789.4-2010 continue to the next step, if the negative direct reporting of results. 370 samples were detected, 5 strains of Salmonella were detected, the standard group and the experimental group test results has been in line with the rate of 100%. The application of Real-time PCR, can provide guidance for the purpose of bacteria, help to improve the detection rate;for PCR Real-time PCR negative samples, can be reported directly to 3-4 days ahead of time, shorten the cycle of the experiment; due to the follow-up only for Real-time PCR positive samples were measured to reduce the workload of the experimenter, saving reagent negative samples follow-up detection.

关 键 词：沙门氏菌 REAL-TIMEPCR 

分 类 号：TS207.4[轻工技术与工程—食品科学]