Puma （Puma concolor） epididymal sperm morphometry  

作　　者：Hernan Cucho Virgilio Alarcon Cesar Ordonez Enrique Ampuero Aydee Meza Caries Solere 

机构地区：[1]Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Peru [2]Functional Biology and Physical Anthropology Department, University of Valencia, C/. Dr Moliner 50, 46100 Burjassot, Valencia, Spain [3]Proiser R+D, S.L., Scientific Park of the University of Valencia, 46980 Paterna, Spain

出　　处：《Asian Journal of Andrology》2016年第6期879-881,共3页亚洲男性学杂志（英文版）

摘　　要：The Andean puma （Puma concolor） has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component （PC） analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.The Andean puma （Puma concolor） has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component （PC） analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.

关 键 词：principal component analysis PUMA sperm morphometry SUBPOPULATION wild animal 

分 类 号：R[医药卫生]