抑菌试验中抑菌圈法的比较研究  

Study on the Inhibition Zone Method in Antimicrobial Test

作　　者：谭才邓[1] 朱美娟[1] 杜淑霞[1] 姚勇芳[1] 

机构地区：[1]广东轻工职业技术学院,广州510300

出　　处：《食品工业》2016年第11期122-125,共4页The Food Industry

基　　金：广州市科技计划项目(201510010113);创新强校工程专项资金项目(2A20301)

摘　　要：试验比较研究了抑菌圈法中的K-B法、牛津杯法和打孔法在抑菌试验中的优缺点,结果表明：打孔法操作简单、方便快捷,获得的抑菌圈质量高、重复性好,是优先选用的方法;牛津杯法获得的抑菌圈质量较高,重复性较好;K-B法则只适宜进行定性试验。在进行抑菌圈法试验时,建议使用预加菌液倾注平板法来制备试验平板,试验平板中菌体浓度应在1×10^5-1×10^6 CFU/m L,试验药物应选择适宜的浓度,加入试验药物后应在4℃条件下预扩散2h左右再放置培养箱进行培养,另外还应考虑培养基的pH。Three methods of inhibition zone test such as K-B method, oxford cup method and beating hole method were compared in this study. The results showed that the beating hole method was simple, convenient and reproducible, the inhibition zone obtained from this method was high quality; The oxford cup method was reproducible and its inhibition zone was high quality too; However, the K-B method was just suited for qualitative tests. Pour plate method was that pre-adding microbe was suggested to use for preparing experimental plate when.performing the antimicrobial test, and the cell concentration of the plate should be reached from 1 × 10^5 CFU/mL to 1× 10^6 CFU/mL. The concentration of the test drug should be appropriate, the plate should be placed in 4 ℃ fridge for pre-diffusing after added test drug. In addition, the pH of culture medium should be considered.

关 键 词：抑菌试验 抑菌圈 K-B法 滤纸片法 牛津杯法 打孔法 

分 类 号：R446.5[医药卫生—诊断学]