HS-SPME-GC-MS法快速检测积雪草挥发性成分  

Rapid Detection of Volatile Constituents in Centella asiatica by HS-SPME-GC-MS

作　　者：张伟[1] 张娟娟[1] 尹震花[1] 康文艺[1] 

机构地区：[1]黄河科技学院医学院,郑州450063

出　　处：《中国药房》2016年第33期4710-4711,共2页China Pharmacy

基　　金：河南省科学技术厅科学技术发展计划(No.142102310147;152102310171);郑州市科学技术局科技发展计划(No.20140790)

摘　　要：目的：建立快速检测积雪草挥发性成分的方法。方法：采用顶空固相微萃取-气相色谱-质谱联用法（HS-SPME-GC-MS）。色谱条件：色谱柱为HP-5 MS石英弹性毛细管柱,载气为高纯氦气（99.999%）,流速为1.0 ml/min,进样口温度为250℃,程序升温,分流进样,分流比为10：1。质谱条件：电离方式为电子轰击离子源,电离能量为80 e V,离子源温度为230℃,四极杆温度为150℃,传输线温度为280℃,电子倍增器电压为1 494 V,扫描范围为m/z 30-400。以面积归一化法测定各挥发性成分的相对含量。结果：从积雪草中鉴定出16个挥发性成分,占总峰面积的79.22%,其主要挥发性成分为（E）-β-金合欢烯（36.97%）、石竹烯（13.61%）、大根香叶烯D（10.95%）、α-菖蒲二烯（2.76%）和香树烯（2.33%）。结论：该研究基本明确了积雪草的主要挥发性成分,为进一步认识积雪草提供了一定科学依据。OBJECTIVE： To establish a method for the rapid detection of the volatile constituents in Centella asiatica. METH- ODS： HS-SPME-GC/MS was adopted, the chromatographic conditions： column was HP-5 MS fused silica capillary column, carri- er gas was high-purity helium （99.999%） at a flow rate of 1.0 ml/min, inlet temperature was 250 ℃ （temperature programmed）, split injection, split ratio was 10： 1. MS conditions： ionization was electrospray ionization source, ionization energy was 80 eV, ion source temperature was 230 ℃, quadrupole temperature was 150 ℃, the transmission line temperature was 280 ℃, electron multiplier voltage was 1 494 V, and scan range was m/z 30-400. Retention index method was combined with peak area normalization method to determine the relative percentage contents of each volatile constituent. RESULTS： Totally 16 volatile constituents were identified from C. asiatica, accounting for 79.22% to the total peak area, the main volatile constituents were （E） -β- fame- sene （36.97%）, caryophyllene （13.61%） ,germacrene D（10.95% ）, α-acoradiene （2.76%） and （-）-alloaromadendrene （2.33%）. CONCLUSIONS ： The study basically clears the main volatile constituents of C. asiatica, and provides certain scientific basis.

关 键 词：积雪草 挥发性成分 快速检测 顶空固相微萃取 气相色谱-质谱联用法 

分 类 号：R284.1[医药卫生—中药学]