牛磺酸对裸鼠乳腺癌模型肿瘤生长的影响及其机制  

Effects of taurine on tumor growth in a nude mouse breast cancer model

作　　者：宋玉美[1] 贺瑜[1] 梁柏莹 郭松超[1] 欧阳轶强[1] 

机构地区：[1]广西医科大学公共卫生学院营养与食品卫生学教研室,南宁530021

出　　处：《中国癌症防治杂志》2016年第5期269-272,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西科技基础条件平台建设项目资助(14-91-02)

摘　　要：目的探讨牛磺酸对裸鼠乳腺癌模型肿瘤生长的影响及机制。方法 10只SPF级5周龄裸鼠接种MDA-MB-231乳腺癌细胞后随机分为牛磺酸干预组和对照组,每组5只。牛磺酸干预组给予添加5%的牛磺酸饮水,对照组给予正常无菌饮水。测量两组小鼠肿瘤体积和肿瘤质量,采用蛋白免疫印迹法检测雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、人类表皮生长因子受体2(human epidemic growth factor receptor2,HER-2)和鸟苷三磷酸酶激活蛋白(guanosine triphosphatase activating protein,p21ras)表达量的变化。结果实验结束时所有裸鼠均存活,裸鼠乳腺癌模型造模成功。牛磺酸对裸鼠乳腺癌模型肿瘤的抑制率为41.6%。相对于对照组,牛磺酸干预组裸鼠肿瘤组织中ER表达量提高了134%(P<0.05),PR表达量提高了47.5%(P<0.05),HER-2表达量降低了67%(P<0.05),p21ras表达量降低了62.2%(P<0.01)。结论牛磺酸可抑制裸鼠乳腺癌模型肿瘤生长,其机制可能与上调ER、PR和下调HER-2、p21ras有关。To study the effect of taurine on the humanized nude mouse model of triple-negative breast cancer. Methods Five-week-old SPF level nude mice（n=10） were inoculated with MDA-MB-231 breast cancer cells and randomly divided into one group that received normal drinking water and another group that received water containing 5% taurine（n=5 animals per group）. Tumor volume and weight were examined after treatment, and Western blotting was used to assess expression of estrogen receptor （ER）, progesterone receptor（PR）,human epidermal growth factor receptor 2 （HER-2）and guanosine triphosphatase-activating protein p21ras. Results All mice survived until the end of the experiment,and all tumors grew. Compared to control mice, mice given taurine had tumors with 41.6% smaller weight and volume（P〈0.05）,134% higher expression of ER（P〈0.05）, 47.5% higher expression of PR （P〈0.05）,67% lower expression of HER-2（P〈0.05）and 62.2% lower expression of p21ras（P〈0.01）. Conclusion Taurine inhibits tumor growth by up-regulating expression of ER and PR and down-regulating expression of HER-2 and p21ras.

关 键 词：乳腺肿瘤 牛磺酸 抗肿瘤 裸鼠 雌激素受体 孕激素受体 人类表皮生长因子受体2 鸟苷三磷酸酶激活蛋白 

分 类 号：R737.9[医药卫生—肿瘤]