固相萃取-高效液相色谱法同时测定番茄酱中4种苏丹红染料  

Simultaneous determination of four kinds of tonyred in ketchup by SPE-HPLC

作　　者：陈波[1] 

机构地区：[1]宁波市北仑区疾病预防控制中心检验科,浙江宁波315800

出　　处：《中国生化药物杂志》2016年第10期152-154,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的建立固相萃取-高效液相色谱法同时测定番茄酱中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ。方法样品用乙腈∶三氯甲烷（v/v）=3∶1提取,经Pro Elut SDH固相萃取柱净化,以乙腈和0.2%磷酸溶液为流动相,经梯度洗脱分离,在500nm波长下进行检测,外标法定量。结果苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ在0.1-20μg/m L浓度范围内的加标回收率在83.4%-94.6%,其相对标准偏差（RSD）为2.9%-5.4%,在0.1-20.0 mg/L范围呈现良好的线性,其回归系数大于0.999,检出限（LOD）分别为苏丹红Ⅰ4μg/kg、苏丹红Ⅱ8μg/kg、苏丹红Ⅲ4μg/kg、苏丹红Ⅳ5μg/kg。结论该方法简便、灵敏、重现性好,能满足番茄酱中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的检测需要。Objective To establish simultaneous determination of tonyred Ⅰ,Ⅱ,Ⅲ and Ⅳ in ketchup by solid phase extraction-high performance liquid chromatography（ SPE-HPLC）. Methods Samples were extracted by solvent of acetonitrile： trichloromethane（ v / v） = 3 ∶1,and purified by Pro Elut SDH. The acetonitrile and 0. 2% phosphoric acid solution as the mobile phase with gradient elution separation,the detection wavelength was500 nm,quantified by external standard method. Results Tonyred I,II,III,IV recoveries in 0. 1- 20 g / m L concentrations ranged at the rate of 83. 4%-94. 6%,the relative standard deviation（ RSD） ranged from 2. 9% to 5. 4%,showed a good linear range of 0. 1- 20. 0 in mg / L,the regression coefficient was greater than 0. 999,the limit of detection（ LOD） respectively as tonyred I 4 μg / kg,tonyred II 8 μg / kg,4 μg / kg tonyred tonyred III,IV5 μg / kg. Conclusion The method is simple,sensitive,reproducible,can meet the tonyred I,II,III,IV requires detection of residues.

关 键 词：高效液相色谱 固相萃取 番茄酱 苏丹红染料 

分 类 号：TQ617.1[化学工程—精细化工] R155.51[医药卫生—营养与食品卫生学]