成都市售辣椒粉霉菌污染调查及实时荧光定量PCR法在其中的应用  

Survey of mould contamination in paprika powder and the application of rapid detection for common mycotoxin-producing moulds with nonmodified magnetic beads-multiple QPCR

作　　者：凌莉[1,2] 陈宇航[1] 王溯源[3] 金燕[4] 颜妍[1] 张立实[1,5] 裴晓方[1,5] LING Li CHEN Yu-hang WANG Su-yuan JIN Yan YAN Yan ZHANG Li-shi PEI Xiao-fang(School of Public Health, Sichuan University, Chengdu, Siehuan 610041, China)

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]广东省珠海市疾病预防控制中心,广东珠海519060 [3]四川省疾病预防控制中心,四川成都610041 [4]浙江中医药大学,浙江杭州310053 [5]四川省食品安全监测与风险评估重点实验室,四川成都610041

出　　处：《现代预防医学》2016年第22期4156-4158,4195,共4页Modern Preventive Medicine

基　　金：国家自然科学基金重点项目"水产品/农产品/香料中化学污染物/产毒真菌的快速检测和食品中有害物质健康危害的安全性评价研究"(No.81030053)资助

摘　　要：目的了解成都地区市售辣椒粉受霉菌污染的状况,探讨裸磁珠-多重实时荧光定量PCR方法快速定量检测霉菌数量的实用性。方法于成都市区随机选取农贸市场,采集辣椒样品40份,按照国标GB4789.15-2010对样品进行霉菌计数,纯化后以镜下特征及菌落特征进行菌种鉴定及分类计数,同时采用裸磁珠-实时荧光PCR方法检测霉菌数量,通过与传统方法比较,探讨裸磁珠-实时荧光PCR方法的实用性。结果所采集样品的霉菌污染均数达到了5.1×103CFU/g;对曲霉属、青霉属及镰刀菌属分类计数,裸磁珠-多重实时荧光定量PCR的检测法计数结果几何均数分别为6.1×108、4.0×101及2.9×106CFU/g,此结果与传统培养计数法2.0×103、1.1×102及2.1×101CFU/g相比有显著增加,两者结果具有统计学差异。结论成都市售辣椒粉受霉菌污染严重,对消费者健康构成较大威胁。相比传统培养法,裸磁珠-多重实时荧光定量PCR方法节约时间、灵敏度高且受稀释等操作的影响较小、受样本成分及样本中其他霉菌干扰较小,具有很高的应用价值。Objective This work was to investigate the mould contamination level in paprika powder sold in the downtown of Chengdu city, and to explore and evaluate the application value of non-modified magnetic beads-multiple QPCR method. Methods 40 paprika powder samples were randomly collected from farmers＇ markets, and examined for mould counts based on the method of GB4789.15-2010. The structure of mould was observed with microscope to indentify the mould＇s species. Meanwhile, these samples were also analyzed by the previously-established non-modified magnetic beads-multiple QPCR method. Results The average mould counts in paprika samples was as high as 5.1×10^3 CFU/g. The identification and counting of Aspergillus, Penicillium and Fusarium were conducted.The geometric mean of the counts by the non-modified magnetic beads-multiple QPCR method was 6.1×10^8, 4.0×10^1, 2.9×10^6 CFU/g for Aspergillus, Penicillium and Fusarium, respectively. The result of the traditional colony-counting method was 2.0×10^3,1.1 ×10^2 and 2.1 ×10^1 CFU/g. There was statistically significant difference between the two counting methods. Conclusion The spices sold in Chengdu＇s farmer＇s market is seriously contaminated by mould. Non-modified magnetic beads-multiple QPCR has high application value since it is less affected by operations andby other mold in the samples, and meanwhile with high sensitivity and efficiency.

关 键 词：快速检测 多重实时荧光定量PCR 辣椒样品 霉菌污染 市场调查 

分 类 号：R155.5[医药卫生—营养与食品卫生学]