裂殖壶菌高密度发酵生产DHA  

Production of Docosahexaenoic Acid by High Cell Density Cultivation of Schizochytrium Sp.

作　　者：班甲[1] 陈骏佳[1] 付尽国[1] 李浩洋[1] 班雯婷 Ban Jia Chen Junjia Fu Jinguo Li Haoyang Ban Wenting(Guangdong Provincial Bioengineering Institute （Guangzhou Sugarcane Industry Research Institute）, Guangdong Provincial Key Laboratory of Sugarcane Improvement and Biorefinery, Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Guangdong Provincial Engineering Technology Research and Development Center of Biomass Comprehensive Utilization, Guangzhou Key Laboratory of Biomass Comprehensive Utilization, Guangzhou 510316, China)

机构地区：[1]广东省生物工程研究所(广州甘蔗糖业研究所),广东省甘蔗改良与生物炼制重点实验室,广东省生物质高值化利用工程实验室,广东省植物纤维综合利用工程技术研究开发中心,广州市植物纤维综合利用重点实验室,广东广州510316

出　　处：《广东化工》2016年第22期63-66,共4页Guangdong Chemical Industry

基　　金：广东省公益研究与能力建设专项(2014A010107016)

摘　　要：本实验从海水中筛选分离了一株裂殖壶菌,通过摇瓶实验对其发酵产二十二碳六烯酸的培养基、发酵参数进行了优化,在此基础上对裂殖壶菌进行了50 L发酵罐流加补料发酵,并成功放大培养到500 L发酵罐。在500 L发酵罐经过96 h的培养,生物量、油脂含量和二十二碳六烯酸含量分别达到110.7、43.5、22.1 g/L,初步达到了工业化生产的水平。In the study, a Schizochytrium sp. strain was isolated from the sea. The medium compositions and culture conditions in shake flasks for production of DHA by Schizochytrium sp. was optimized. On these bases, a fed-batch fermentation ofSchizochytrium sp. in a 50 L bioreactor was carried out, and successfully scaled up in a 500 L bioreactor. The final biomass, oil productions and DHA yield were 110.7, 43.5, 22.1 g/L, respectively. The results preliminarily achieved the level of industrial production.

关 键 词：裂殖壶菌 二十二碳六烯酸 补料流加 放大培养 

分 类 号：TQ929[轻工技术与工程—发酵工程]