羊肺蛋白酶解工艺优化及体外抗氧化活性研究  

Optimization of protease enzymatic hydrolysis of sheep lung and studies on antioxidant activities of its hydrolysates

作　　者：杨旭卉 文飞[1] 王先倩 齐琦[2] 苏伟[1] 托尼 YANG Xu-hui WEN Fei WANG Xian-qian QI Qi SU Wei TUO Ni(College of Liquor and Food, Guizhou University, Guiyang, Guizhou 550025, China College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China Qinglong County, Guizhou Sea Power Sheep Halal Food Processing Ltd., Southwest Guizhou Autonomous Prefecture, Guizhou 561400, China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州省晴隆县海权清真肉羊食品加工有限公司,贵州黔西南布依族苗族自治州561400

出　　处：《食品与机械》2016年第10期151-157,共7页Food and Machinery

基　　金：黔西南州种草养羊产业发展省州科技合作专项项目(编号:黔西南科号[2012]4)

摘　　要：为优化羊肺酶解工艺及探讨酶解物体外抗氧化活性,采用中性蛋白酶酶解羊肺,以水解度为评价指标,在单因素试验的基础上,运用响应面法优化酶解工艺。结果表明:羊肺的最佳酶解条件为酶解温度50℃,加酶量1 700U/g,水料比2∶1(mL/g),酶解时间4h。在最优条件下,进行3次验证性实验,测得实际水解度为18.89%,与理论水解度(18.19%)相比误差较小。体外抗氧化结果表明:羊肺酶解产物具有一定的抗氧化活性,其还原力的吸光值为0.472,清除DPPH·、·O_2^-和·OH的IC_(50)值分别为67.00,40.07,31.88mg/mL。By using the hydrolysis degree as the evaluation, the sheep lung enzymatic process was optimized to investigate the antioxidate activities of the enzymolysis products, hydrolyzed by neutral protease. On the basis of single factor studies, the response surface analysis （RSM） assay was then used to obtain the best enzymatic proscess. Our results showed that the optimal condition was as fol- lows. The enzyme amount was 1 700.0 U/g, with solid-liquid ratio 2 ： 1（mL/g） , enzymatically hydrolyzed for 4 h at 50 ℃. Under this optimal conditions, 3 times of the replication experiments turned out that the error of the actual degree of hydrolysis （ 18.89 % ） was small, compared with the theory one （18.19 % ）. Moreover, the absorption value of reducing power was found to be 0.472, and IC50 of the DP- PH radical scavenging rate, the superoxide anion radical scavenging rate, and the hydroxyl radical scavenging rate were 67 mg/mL,40.07 mg/mL, and 31.88 mg/mL, respectively. These results of a- bove detection indicated that enzymatic hydrolysates of sheep lungs possessed certain antioxidant activity in vitro.

关 键 词：羊肺 蛋白 酶解 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]