小麦-簇毛麦易位系中贮藏蛋白的鉴定及分析  

Identification and Analysis of Storage Protein in Chinese Spring(CS)-Dasypyrum villosa Translocation Lines

作　　者：刘皋芃 高翔[1,2] 杨明明[1,2] 赵万春[1,2] 董剑[1,2] 石引刚[1,2] 陈良国[1,2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]陕西省小麦新品种培育工程研究中心,陕西杨凌712100

出　　处：《西北农业学报》2016年第9期1304-1310,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十二五"农村领域国家科技计划项目(2011AA100501);国家现代农业产业技术体系建设专项(CARS-3-2-47);国家自然科学基金(31171538;30900896);中央高校基本科研业务费专项(QN2009007)~~

摘　　要：为探讨簇毛麦染色体对小麦品质的影响,利用A-PAGE、SDS-PAGE和高效毛细管电泳(High-performance capillary electrophoresis,HPCE)技术对12个易位系及7个附加系的贮藏蛋白进行鉴定。将簇毛麦的一条ω-醇溶蛋白基因定位于1VL。结果表明:易位系T1DL·1VS可用来改良小麦品质;定位于簇毛麦1VS染色体上的高分子谷蛋白在小麦中表达不稳定;其他材料醇溶蛋白组成发生改变较小,可能对面筋相关品质影响不大,在育种工作中不会引起面筋相关品质下降;易位系材料中的小麦醇溶蛋白的质量分数有所增加。For studying the effects of Dasypyrum villosa chromsomes on grain processing quality of Chinese Spring（CS）, A-PAGE SDS-PAGE and HPCE were used to analyze to identify the seed storage protein of 12 translocation lines and 7 addition lines in this paper. One of the oo-Gliadin was located in the 1VL,a-Gliadin located in the 1VS,4VS and 6VL. The results showed that the translocation lines TIDL · 1VS could improve wheat quality. Expression level of the HMW-GS gene Glu-V1 of D. villo- sum which located on 1VS was low. The gliadin contents of other translocation lines changed a little, and it maybe had little effect on gluten quality, so it cannot decrease related gluten quality in wheat breeding, the gliadin content of wheat in translocation lines increased.

关 键 词：簇毛麦 易位系 贮藏蛋白 小麦品质 

分 类 号：S512.1[农业科学—作物学]