高浓度发酵废水生产单细胞蛋白的菌种筛选  

Strains screening for producing single cell protein from high concentration fermentation wastewater

作　　者：周媛[1] 周子洋 封莉[1] 程翔[1] 张立秋[1] ZHOU Yuan ZHOU Ziyang FENG Li CHENG Xiang ZHANG Liqiu(College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China)

机构地区：[1]北京林业大学环境科学与工程学院,北京100083

出　　处：《环境工程学报》2016年第11期6307-6312,共6页Chinese Journal of Environmental Engineering

基　　金：国家水体污染治理与控制科技重大专项(2013ZX07201007-003)

摘　　要：为了回收高浓度发酵废水中的有用资源,以近平滑假丝酵母(C.parapsilosis)、热带假丝酵母(C.tropicalis)、产朊假丝酵母(C.utilis)、汉逊德巴利酵母(D.hansenli)、酿酒酵母(S.cerevisiae)、皮状丝孢酵母(T.cutaneum)、白地霉(G.candidum)和黑曲霉(A.niger)8种常见工业菌种为研究对象,通过摇瓶发酵,考察了不同菌种利用高浓度发酵废水生产单细胞蛋白(SCP)的能力,同时比较废水灭菌和不灭菌两种条件对SCP产量的影响。结果表明,在废水COD浓度69 600 mg·L^(^(-1))、TN浓度4 048 mg·L^(^(-1))、初始pH 6.5、28℃、150 r·min^(-1)、灭菌条件下发酵40 h,C.parapsilosis的菌体生物量和粗蛋白含量最高,可达7.04 g·L^(-1)和1.87 g·L^(-1),对废水中COD和TN的去除率分别为33.5%和20.1%;C.parapsilosis和G.candidum可作为废水生产SCP并高效回收氮素和有机质的优势菌种。在不灭菌条件下,各菌种生产SCP的能力明显高于灭菌条件,且不同菌种产量差异不大,生物量和粗蛋白含量平均高达8.0 g·L^(-1)和4.0 g·L^(-1),废水COD和TN的去除率分别为47%和33%。In order to recover valuable resources from high-concentration fermentation wastewater,the single cell protein（ SCP） producing ability of strains using molasses fermentation wastewater were investigated. The study employed eight fungal strains commonly used in industrial applications： Candida spp.（ parapsilosis,tropicalis,utilis）,D. hansenli,S. cerevisiae,T. cutaneum,G. candidum and A. niger. Flask fermentation experiments were used to study the SCP-producing ability of the various strains,and the effect of sterilization on SCP production. Wastewater with initial COD and TN of 69 600 mg·L^（-1）and 4 048 mg·L^（-1）,respectively,was fermented under sterile conditions of pH 6. 5,temperature 28 ℃,and 150 r·min^（-1）for 40 h. The results showed that C. parapsilosis had the highest biomass dry weight（ 7. 04 g·L^（-1）） and protein content（ 1. 87 g·L^（-1））,and achieved COD and TN removal rates of 33. 5% and 20. 1%,respectively. C. parapsilosis and G. candidum could be used as the predominant strains for SCP production as well as nitrogen and organic matter high efficient recovery from wastewater. When fermentation wastewater was pretreated without sterilization,the SCP production of strains was significantly higher than when sterilized. In addition,no significant differences in SCP production were observed between strains. The average biomass dry weight,protein content,COD,and TN removal of strains were as high as 8. 0 g·L^（-1）,4. 0 g·L^（-1）,47%,and 33%,respectively.

关 键 词：高浓度发酵废水 酵母菌 霉菌 单细胞蛋白 

分 类 号：TQ939.9[化学工程]