固芯液化技术对微囊化Penicillium purpurogenum Li-3细胞使用性能的影响  

The effect of solid core liquefaction technology on microencapsulated Penicillium purpurogenum Li-3 cells performance

作　　者：蔡静晓 曹红[1] 叶海[2] 罗婷[1] 杨露[1] 王娜[1] 刘进[1] 刘瑞麟[1] 李春[3] 

机构地区：[1]嘉兴学院生物与化学工程学院,嘉兴314001 [2]石河子大学化学化工学院,石河子832000 [3]北京理工大学生命学院,北京100081

出　　处：《中国食品添加剂》2016年第10期69-76,共8页China Food Additives

基　　金：浙江省大学生科技创新活动计划(新苗人才计划)项目资助(2015R417008);国家自然科学基金项目"液芯微囊化Penicillium purpurogenum Li-3细胞的制备及其催化性能研究"(21266029)

摘　　要：以微囊化产紫青霉细胞(Penicillium purpurogenum Li-3)催化甘草酸(GL)定向合成具有更高生物利用度、甜度以及安全性的单葡萄糖醛酸基甘草次酸(GAMG)为目的,研究固芯液化技术对微囊化细胞使用性能的影响。结果显示,液芯微囊化细胞的生长情况和传质性能明显优于固芯微囊化细胞,并在第1批次催化反应中,液芯微囊化细胞的相对活性达到98.7%,比固芯微囊化细胞(72.7%)要高。9批次重复利用实验表明,与固芯微囊化细胞相比,液芯微囊化细胞的相对活性较高,而机械强度和操作稳定性要稍显逊色。此外,固芯液化的技术与条件对微囊化细胞发挥最佳催化效果至关重要。The effect of solid core liquefaction of microencapsulated penicillium purpurogenum Li-3 cells on its performance of improving hydrolysis of glycyrrhizin （GL） into glycyrrhetinic acid monoglucuronide （GAMG） to have better bioavailability, sweetness, and security was investigated in this paper. The results showed that the mass transfer performance and growth of alginate-chitosan liquid core microencapsulated cells was obviously better than solid core microencapsulated cells. The first batch of catalytic reaction, compared with solid core microencapsulated cells （72.7%）, the relative catalytic activity （98.7%） of liquid core microencapsulated cells was higher. But the mechanical strength and repeated useage of liquid core microencapsulated cells weren＇ t as good. In addition, the key techniques and technological conditions used for solid core liquefaction of microencapsulated cells was crucial on the catalytic effect.

关 键 词：固芯液化技术 微囊化Penicillium purpurogenum Li-3细胞 使用性能 甘草酸 单葡萄糖醛酸基甘草次酸 

分 类 号：Q939.5[生物学—微生物学]