脂肪酶TL IM/K_2CO_3催化Markovnikov加成反应研究  

The Markovnikov addition catalyzed by lipase TL IM from Thermomyceslanuginosus/K_2CO_3

作　　者：杜理华[1] 杨文俊[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《浙江工业大学学报》2016年第6期649-653,共5页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(21306172);国家948计划资助项目(2014-4-29);浙江省自然科学基金资助项目(LY17B020010);浙江省公益农业计划项目(2014C32094)

摘　　要：咪唑类化合物因其具有广泛的生物活性在医药行业中占有重要的地位.实验首次采用了一种脂肪酶lipase TL IM(Thermomyces lanuginosus)/K_2CO_3催化的咪唑类化合物与乙烯酯类化合物的Markovnikov加成反应新方法.系统研究了催化剂lipase TL IM/K_2CO_3、溶剂、底物结构、底物摩尔比、温度及反应时间对Markovnikov加成反应的影响规律.实验结果表明:咪唑类化合物与乙烯酯类化合物发生Markovnikov加成反应的最佳条件为脂肪酶lipase TL IM/K_2CO_3(K_2CO_3质量分数为4.5%),底物摩尔比1∶6,温度为50℃且反应时间为24h,并在此条件下反应产率高达85%.Because of the widely biological actives, imidazole compounds play an important role in the pharmaceutical industry. The Markovnikov addition of imidazole derivatives and vinyl esters catalyzed by lipase TL IM from Thermomyces lanuginosus/K2CO3 was first reported here. The influence of the amount of catalyzes, solvent, the structure of substrate, temperature and the reaction time were explored systematically. The performence of Markovnikov was better when it reacted for 24 h at 50 ℃ and was catalyzed by lipase TL IM /K2CO3 （K2CO3 ： TL IM /K2CO3, 4.5%, m/m） and the molar ratio of substrate was 1： 6. About 85% conversion can be finally obtained.

关 键 词：Markovnikov加成 咪唑类化合物 酶促反应 脂肪酶 

分 类 号：R944[医药卫生—药剂学]