激活核受体PXR促进小鼠GM-CSF来源的树突状细胞的分化  

Activation of PXR promotes differentiation of mouse GM-CSF derived dendritic cells

作　　者：邱怀娜[1] 查贺飞 曲佳乐[1] 王梅[1] 李璐[1] 齐艳伟 黄俊[1] 杨权(指导)[1] 

机构地区：[1]广州医科大学基础学院病原生物学与免疫学教研室,广州医科大学中法霍夫曼免疫研究所,广州511436

出　　处：《中国免疫学杂志》2016年第11期1593-1597,共5页Chinese Journal of Immunology

基　　金：广州医科大学科学科研项目资助(2015C01)

摘　　要：目的:研究孕烷受体(PXR)激动剂pregnane-16α-carbonitrile(PCN)对小鼠GM-CSF来源树突状细胞(DC)分化的影响。方法:建立小鼠DC体外GM-CSF和IL-4诱导分化体系,并用PCN对体系进行处理。流式细胞术检测CD11c+DC的比例;ELISA和流式细胞术检测DC产生细胞因子IFN-γ、IL-1、IL-2和IL-12的量;实时荧光定量聚合酶链反应(Real-time RT-PCR)检测调控DC分化相关信号通路基因的表达。结果:成功建立小鼠DC体外分化体系;PCN处理能显著促进DC分化,且DC产生的细胞因子IFN-γ和IL-2的量显著增多;Real-time RT-PCR检测结果表明,与对照组相比,PCN处理组中调控DC分化的Notch和Wnt信号通路相关基因HES1、WISP1和WISP2的表达量显著上升。结论:PXR激动剂PCN能显著促进小鼠GM-CSF来源的DC的分化及其IFN-γ和IL-2的产量,并且这种促进作用可能是通过Notch和Wnt信号通路进行调控。Objective： To investigate the influence of Pregnane X Receptor（ PXR） agonist pregnane-16α-carbonitrile（ PCN）on mouse GM-CSF derived dendritic cell（ DC） differentiation. Methods： Mouse DC differentiation was induced by GM-CSF and IL-4in vitro,and treated with PCN. The proportion of CD11c~＋ DC was measured by flow cytometry; the production of Interferon-γ（ IFN-γ）,Interleukin-1（ IL-1）,Interleukin-2（ IL-2） and Interleukin-12（ IL-12） of DC were analyzed by ELISA and flow cytometry; Real-time RT-RCR was used to detect the expression of genes involved in the signaling pathway which regulated DC differentiation. Results：Mouse DC was successfully induced by GM-CSF and IL-4 in vitro. Treatment of PCN significantly increased the percentage of CD11c~＋ DC and the protein levels of IFN-γ and IL-2 produced by DC were notably enhanced. Real-time RT-RCR results indicated that the expression of Notch and Wnt signaling pathway related genes HES1,WISP1 and WISP2 were up-regulated in PCN treatment group when compared to control group. Conclusion： The PXR agonist PCN can promote the differentiation of mouse GM-CSF derived DC and increased the production of IFN-γ and IL-2 by DC,and Notch and Wnt signaling pathway may play a pivotal role in this process.

关 键 词：树突状细胞 分化 PREGNANE X RECEPTOR NOTCH信号通路 WNT信号通路 

分 类 号：R392.12[医药卫生—免疫学] R392.11[医药卫生—基础医学]