柑橘绿霉病菌木聚糖酶基因(PdXY2)功能初步研究  

Preliminary study on the function of PdXY2 in Penicillium digitatum

作　　者：由书妍 刘红霞 于瑞君 郑丽君 范诗言 李红叶[2] 

机构地区：[1]大连市农业科学研究院,大连116036 [2]浙江大学生物技术研究所,杭州310058

出　　处：《植物保护》2016年第6期95-99,共5页Plant Protection

基　　金：国家自然科学基金(31371961);国家现代农业产业技术体系(CARS-27)

摘　　要：柑橘绿霉病菌(Penicillium digitatum)是引起柑橘储藏期腐烂最主要的病原之一,严重影响了柑橘产业发展。本试验克隆了柑橘绿霉病菌的木聚糖酶基因(PdXY2),对其表达进行研究,在柑橘发病过程中,PdXY2的表达显著升高,至48h达到最高,其表达量为对照的4倍。为进一步明确PdXY2基因功能,构建了PdXY2基因缺失突变株(ΔPdXY2),ΔPdXY2突变株的致病性与野生型相比没有明显差异。由此我们推断,在柑橘绿霉病菌侵染柑橘的过程中PdXY2有一定的作用,但是单一缺失木聚糖酶PdXY2基因不能影响其致病性。Penicillium digitatumis one of the most important pathogen causing green mold disease which impedes the development of citrus industry.In this study,we cloned a xylanase gene PdXY2 and characterized its functions via knock-out strategy.The expression of PdXY2 was up-regulated during the primary stage of infection,and reached a peak at 48 h.However,PdXY2-disrupted mutant（ΔPdXY2）showed no reduction in virulence.Taken together,our results demonstrated that PdXY2 may play an important role in infection and single knock-out of PdXY2 showed no reduction in virulence.

关 键 词：柑橘绿霉病菌 木聚糖酶 基因表达 基因突变 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]