CCNB1在卵巢癌中的表达及意义  

Expression and significance of CCNB1 in ovarian carcinoma

作　　者：李莉[1] 熊国平[1] 颜琳[1] 张庆华[1] 

机构地区：[1]武汉市中心医院妇产科,武汉430000

出　　处：《现代妇产科进展》2016年第11期818-820,824,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨CCNB1在卵巢癌诊断和预后判断的作用。方法:通过Oncomine数据库分析并提取组织RNA,Real-time PCR法检测CCNB1在卵巢癌组织中的表达。通过在线数据库及分析工具"the Kaplan Meier plotter"(KM plotter)数据库的应用发掘CCNB1的预后价值。结果:Oncomine数据库中共有18个研究结果涉及正常卵巢组织和卵巢癌组织中的CCNB1的表达情况,全部显示CCNB1在卵巢癌中表达增高。本研究对9对配对的卵巢癌及其癌旁组织进行Real-time PCR,得到相同结果。在KM plotter数据库中的分析显示:无论是无进展生存期(PFS)或总生存期(OS),CCNB1 mRNA水平越高,卵巢癌患者的预后越差(HR>1,P<0.05)。且CCNB1 mRNA高水平在早于2期(包括2期)卵巢癌患者中提示预后较差,在晚于3期(包括3期)患者中CCNB1 mRNA水平与预后无明显相关性。结论:CCNB1在卵巢癌组织中表达升高,可作为提示预后差的卵巢癌的分子标记物。Objective： To explore the expression and prognostic value of cyclin B1（ CCNB1） in ovarian carcinoma（ OC）. Methods： The mRNA level of CCNB1 in OC tissues and the corresponding normal tissues were obtained from the Oncomine database by Real-time PCR.The prognostic value of CCNB1 was retrieved from an online survival analysis tool ＂the Kaplan Meier plotter＂（ KM plotter） database. Results： In Oncomine database,there were 18 studies of different types involved CCNB1 expression in OC and normal tissues,of which 18 studies showed up-regulated of CCNB1 in OC（ P〈0. 05）. The same result was got from Real-time PCR in 9 pairs of tissues of OC specimens and in the adjacent non-cancerous tissues. In KM plotter database,OC patients with high expression of CCNB1 showed a worse progression-free-survival（ PFS）（ P〈0. 05） and worse overall survival（ OS）（ P〈0. 05）. CCNB1＇s high expression was associated with worsen OS in early stages（ stage I and stage 1 ＋ 2）,while not for late stages（ stage 3 or later）. Conclusions： CCNB1 was highly expressed in OC,which may used as a biomarker predicted poor outcome.

关 键 词：卵巢癌 预后 CCNB1 KM plotter 

分 类 号：R737.33[医药卫生—肿瘤]