大蒜素对TGF-β_1诱导的胰腺癌细胞EMT的抑制作用及其机制  

Inhibitory effect of allicin on TGF-β1-induced EMT of pancreatic cancer cells

作　　者：马笛[1] 贺川[1] 魏媛[1] 马新宇[1] 张忠良[1] 巩平[1] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832000

出　　处：《山东医药》2016年第41期18-21,共4页Shandong Medical Journal

基　　金：上海市胰腺疾病重点实验室开放课题(PC-2013-02)

摘　　要：目的探讨大蒜素对TGF-β1诱导的胰腺癌细胞上皮-间充质转化(EMT)的抑制作用及机制。方法常规培养胰腺癌细胞株PANC-1细胞,取对数生长期细胞,随机分为大蒜素+TGF-β1组、大蒜素组、TGF-β1组及空白对照组,前三组分别以TGF-β110 ng/m L+大蒜素5μg/m L、大蒜素5μg/m L、TGF-β110 ng/m L处理PANC-1细胞,空白对照组不予干预。应用MTT法检测细胞增殖情况;划痕试验、Transwell小室试验测定胰腺癌细胞的迁移、侵袭能力。RT-PCR法检测各组E-Cadherin、Viminten及NF-κB表达。结果 MTT结果显示大蒜素干预后胰腺癌细胞增殖受到抑制,24 h与48 h的IC50分别为69.44、100.90μmol/L,呈时间和剂量依赖性;划痕试验显示空白对照组、大蒜素+TGF-β_1组、大蒜素组及TGF-β1组8 h迁移率分别为0.56±0.11、0.10±0.07、0.09±0.05、0.61±0.23;24 h迁移率分别为1.00±0.00、0.74±0.20、0.40±0.05、1.00±0.00。Transwell小室试验显示,空白对照组、大蒜素+TGF-β1组、大蒜素组及TGF-β1组的侵袭率分别为2.15±0.04、1.98±0.14、1.01±0.01、2.93±0.02,大蒜素组与其他三组比较差异有统计学意义(P均<0.05);RT-PCR结果显示,与空白对照组相比,TGF-β1组E-cadherin表达明显下调(P均<0.05),Vimentin表达则明显上调(P均<0.05)。与TGF-β1组相比,大蒜素+TGF-β1组及大蒜素组E-candherin表达上调,Vimentin表达下调;同时,NF-κB的表达亦明显下调(P均<0.05)。结论大蒜素能阻断TGF-β1诱导的胰腺癌EMT效应,其可能通过抑制NF-κB信号通路抑制胰腺癌细胞发生EMT。Objective To investigate the inhibitory effects of allicin on TGF-β1 -induced epithelium mesenchymal transition （EMT）of pancreatic cancer cells and its mechanism.Methods The pancreatic cancer cell line PANC-1 in loga-rithmic growth phase were routinely cultured and then were randomly divided into allicin ＋TGF-β1 group,allicin group, TGF-β1 group and the control group.The PANC-1 cells in the former three groups were respectively treated with 10 ng/mL TGF-β1 ＋5 μg/mL allicin,5 μg/mL allicin,and 10 ng/mL TGF-β1 ,and the control group without any treatment.MTT assay was used to detect cell proliferation;scratch test,Transwell chamber experiment were used to measure the migration and invasion of pancreatic cancer cell,and RT-PCR was applied to detect the E-Cadherin,Viminten and NF-κB expres-sion.Results MTT showed that after allicin intervention,the cell proliferation of pancreatic cancer was inhibited,IC50 at 24 and 48 h was 69.44,and 100.9 μmol/L,which was in a time-and dose-dependent manner.The scratch test showed the migration rates at 8 h of the control group,allicin ＋TGF-β1 group,allicin group and TGF-β1 group were 0.56 ±0.11, 0.10 ±0.07,0.09 ±0.05,and 0.61 ±0.23;at 24 h ,they were 1.00 ±0.00,0.74 ±0.20,0.40 ±0.05,and 1.00 ± 0.00.Transwell chamber experiment showed the invasion rates of the control group,allicin ＋TGF-β1 group,allicin group and TGF-β1 group were 2.15 ±0.04,1.98 ±0.14,1.01 ±0.01 and 2.93 ±0.02,and statistically significant difference was found between allicin group and the other three groups （all P 〈0.05）.The RT-PCR showed that compared with the control group,E-cadherin expression was significantly decreased,and Vimentin expression was significantly increased in the TGF-β1 group （all P 〈0.05）.Compared with TGF-β1 group,the E-candherin was up-regulated,Vimentin was down-regulated and the NF-κB expression was also down-regulated in the allicin ＋ TGF-β1 group and allicin group （all P 〈0.05）.Conclusion Allicin has a blocking effect on TGF-β

关 键 词：胰腺癌 大蒜素 上皮间质转化 核因子-ΚB 

分 类 号：R735.9[医药卫生—肿瘤]