HPLC测定动物源性食品中倍他米松的残留  

Determination of betamethasone in animal tissues by high performance liquid chromatography

作　　者：张晶[1] 郭璐[1] 赵兴然 张群[1] 王向红[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《河北农业大学学报》2016年第6期78-82,共5页Journal of Hebei Agricultural University

基　　金：河北省科技支撑项目(152776120D)

摘　　要：为建立检测动物性食品中倍他米松残留的高效液相色谱法。样品经乙酸乙酯提取后,进行液相色谱分析。采用Agilent C18（250mm×4.6mm,5μm）色谱柱,流动相为甲醇：水（60∶40,v∶v）,流速为0.8mL/min,检测波长为240nm,柱温为30℃。方法的检出限和定量限分别为0.152μg/kg和0.502μg/kg。倍他米松在0.5～32μg/mL范围内线性关系良好,相关系数为0.998 9;选取5种动物源性食品进行倍他米松的加标回收试验,回收率在72.73%～93.06%之间,精密度RSD为3.78%（n=6）。本方法简便、可靠、重复性好,可用于动物性食品中倍他米松残留的检测。The research aims to establish a high performance liquid chromatography（ HPLC） method to detect betamethasone in animal tissues. The sample was extracted by ethyl acetate and analyzed by HPLC. An Agilent C18 （250 mm×4. 6 mm, 5 μm）cartridge was used as chromatographic column. The mobile phase was methanol ： water （60 ： 40）, flow rate 0. 8 mL/min, detection wavelength 240 nm and column temperature 30℃. The limit of detection and quantity were 0. 152 μg/kg and 0. 502 μg/kg. The average recovery rate of betamethasone in 5 animal tissues were in the range of 72.73～93.06% with the precision of 3.78% （n=6）. The method is simple,reliable and reproducible,and it is suitable for the determination of betamethasone residue in food of animal tissues.

关 键 词：高效液相色谱 倍他米松 动物性食品 

分 类 号：TS202.3[轻工技术与工程—食品科学]