中高温大曲中高产蛋白酶菌株的选育  

The Breeding of High Protease-producing Strain Isolated from Songhe Daqu

作　　者：李学思 侯小歌[2] 李绍亮 闫培训 张杰 胡炳义[2] LI Xuesi HOU Xiaoge LI Shaoliang LIU Zihong YAN Peixun ZHANG Jie HU Bingyi(Henan Songhe Distillery Co. Ltd., Luyi 477265, China College of Life Science and Agriculture, ZhouKou Normal University, ZhouKou 466000,China)

机构地区：[1]河南省宋河酒业股份有限公司,河南鹿邑477265 [2]周口师范学院生命科学与农学学院,河南周口466000

出　　处：《酿酒》2016年第6期45-49,共5页Liquor Making

基　　金：河南省教育厅科学技术研究重点项目(编号14A180026);周口师范学院科研成果孵化专项基金资助项目(项目编号2011-zknufh-01)

摘　　要：从宋河大曲中共分离得到39株产蛋白酶芽孢菌株,采用透明圈法和摇瓶发酵法筛选出1株高产蛋白酶菌株MSPN-11,酶活达114.8U/m L。以该菌株为出发菌种,采用紫外诱变的育种方法,以致死率和蛋白酶活力为指标选育高产蛋白酶菌株。研究表明:紫外照射时间160s、照射距离50cm^55cm的剂量处理,菌株致死率达80%以上,且90%诱变菌株的R/r值在3.0以上,得到1株高产蛋白酶菌株MSPR8,酶活提高了26.38%。39 protease-producing bacillus isolated from Songhe Daqu were obtained. The strain MSPN-11 which demonstrated high protease productivity（114.8U/m L） was screened by observing transparent rings and measuring the protease activity of shake flask fermentation. The high protease-producing strain would be breeded using ultraviolet mutagenesis with protease activity and lethality rate of colonies as measuring indexes.The results showed that： lethality rate of colonies reached above 80% when UV irradiation time and distance was chose 160 s and 50cm-55 cm, respectively. 90% of the mutant strain R/r values were above 3.0. Strain MSPR8 which showed higher protease activity increasing 26.38% was obtained by screening strains breeded using ultraviolet mutagenesis.

关 键 词：大曲 蛋白酶芽孢菌株 紫外诱变 选育 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]