温度对裙带菜幼孢子体抗氧化系统的影响  

Effect of Temperature on the Antioxidation System of Juvenile Undaria pinnatifida Sporophyte

作　　者：宫庆礼[1] 王鹏[1] 王晓艳[1] 刘岩[1] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2016年第11期91-98,共8页Periodical of Ocean University of China

基　　金：国家海洋局公益性项目(201405040-4)资助~~

摘　　要：以裙带菜(Undaria pinnatifida)幼孢子体为材料,测定了不同温度(12、20、24和28℃)培养下,藻体抗氧化系统变化情况。与对照组(12℃)相比,高温会导致活性氧含量上升,并伴随藻体抗氧化酶活性和抗氧化物含量的变化。当裙带菜处于12℃时,机体总抗氧化能力保持恒定。当遭受较轻程度高温环境时(20℃),藻体ROS平衡被打破,机体内O2-、H2O2等活性氧物质迅速积累,36h达到峰值,同时抗氧化系统中的SOD活性上升,将O2-转化为H2O2。由于损伤较轻,藻体仅依靠原有的抗氧化力及9h内大量合成的AsA缓慢清除H2O2。CAT和POD活性以及GSH含量未见上升。当温度损伤增加时(24℃),为了尽快修复藻体,POD活性3h内急剧上升,同时GSH和AsA迅速合成,在较短时间内控制H2O2含量。随后CAT活性逐渐上升,完成后期修复工作。若温度超过藻体耐受限度(28℃),将造成多种抗氧化酶失活。36h内MDA大量积累,质膜通透性降低,细胞内ROS与抗氧化物外流,最终导致藻体死亡。结果表明,抗氧化系统调节对于裙带菜幼孢子体适应不同环境温度起到了重要作用。这种依机体损伤程度而改变的抗氧化机制,可以更快速、更节能、更有效地修复藻体所受损伤。本结果为进一步解释裙带菜广泛分布原因、研究裙带菜应对不适环境条件机制及培育耐高温品系裙带菜提供一定科学依据。To determine the effect of temperature on the capacity of antioxidation of Undaria pinnatifida,content of ROS,activity of antioxidant enzymes and content of antioxidants were measured in juvenile sporophyte of U.pinnatifida at different temperatures（12,20,24 and 28 ℃）.The results showed that,high temperatures（20,24 and 28 ℃）increased ROS and changed the activity of diverse antioxidant enzymes and content of antioxidants in comparison with the control temperature（12℃）.The total antioxidant capacity of U.pinnatifida remained constant at appropriate temperature（12 ℃）.When exposured at a slighter degree of high temperature（20 ℃）,the content of O_2-and H_2O_2 in organism increased rapidly and reached the maximum at 36 h,while the activity of SOD raised at the same time,to transform O_2-into H_2O_2.Although activity of CAT and POD and content of GSH kept almost constant,H_2O_2 was eliminated slowly by the increased AsA and the basic antioxidant capacity of organism.When temperature rose to 24℃,the damage was increased.Besides SOD,the activity of POD rose dramatically in a short period of time,accompanying with the accumulation of GSH and AsA.Therefore the content of H_2O_2 was kept in a normal level.If the temperature exceeded the tolerance limit of U.pinnatifida（28 ℃）,all of activity of studied antioxidant enzymes decreased.MDA accumulated in 36 h,which can destruct the plasma membrane permeability and lead to alga death eventually.The results of this study illustrated the important role of antioxidation system regulation in the adaptation to different ambient temperatures of young U.pinnatfida sporophyte.Consequently,the study provided some references to explain why this specie is widely distributed,investigate the adaptive mechanism of U.pinnatfida to adverse environmental conditions and breed high temperature tolerant strains.

关 键 词：裙带菜 幼孢子体 温度 抗氧化系统 

分 类 号：S917.3[农业科学—水产科学]