云南小粒速溶咖啡中百菌清提取净化方法研究  

Stndy on Extraction and Purification Method of Chlorothalonil in Yunnan Small Grain Instant Coffee

作　　者：刘丹丹[1] 孙晓帅[1] 王志洪 乔琴[1] 何飞飞[1] 

机构地区：[1]云南大学农学院,云南昆明650091 [2]云南三标农林科技有限公司,云南昆明650302

出　　处：《安徽农业科学》2016年第32期92-93,99,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省建立农科教相结合新型农业社会化服务体系试点云咖啡产品单元子项目"咖啡产品质量标准研究和检测平台"(2014NG004)

摘　　要：[目的]探讨云南小粒速溶咖啡中百菌清的最佳提取净化方法。[方法]分别采用石油醚-丙酮萃取法、固相吸附法及二氯甲烷-丙酮萃取法这3种不同的方法提取净化云南小粒速溶咖啡中的百菌清,并利用高效液相色谱进行加标回收测定,根据回收率的大小和净化方式的繁简,确定最适宜的百菌清测定方法。[结果]3种不同方法提取净化百菌清的加标回收率分别为85.3%、111.4%和118.7%。[结论]通过回收率及净化方式的比较可知,可利用固相吸附法对云南小粒速溶咖啡中的百菌清进行提取纯化。[Objective] The aim was to explore the optimal extraction and purijication method for chlorothalonil in Yunnan small grain instant coffee. [Method] Three different extraction and purification methods（ aether petrolei-acetone extraction,solid-phase adsorption and dichloromethane-acetone extraction） were performed to extract chlorothalonil in instant coffee. Then all of the extractions were tested using high performance liquid chromatography（ HPLC）. The most suitable method was determined according to the percentages of recovery measurement.[Result]The results obtained by three different extraction and purification methods showed that the recovery percentages were 85. 3%,111.4% and 118. 7%,respectively. [Conclusions] By comparing recovery rate and purification methods,solid-phase adsorption method was the best one for testing chlorothalonil in Yunnan small grain instant coffee.

关 键 词：速溶咖啡 百菌清 提取净化 方法优化 

分 类 号：S571.2[农业科学—作物学]