脂蛋白相关磷脂酶A2基因A379V和T403C位点多态性与冠心病的关系研究  

Relationship among lipoprotein-associated phospholipase A2 gene A379V and T403V locus polymorphisms and coronary heart disease

作　　者：林赛梅[1] 赖力[1] 卢华珍 沈晓丽[1] 陈点[1] 王尧城 余鸿[1] 刘尚龙[1] 

机构地区：[1]福建省心血管病重点实验室,福建医科大学省立临床医学院,福建福州350001

出　　处：《心血管康复医学杂志》2016年第6期568-573,共6页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:研究脂蛋白相关磷脂酶A2(Lp-PLA2)基因A379V、T403C位点多态性与冠心病(CHD)遗传易感性的关系。方法:采用基因测序技术检测160例冠脉造影证实的CHD患者(CHD组)及117例健康对象(健康对照组)Lp-PLA2基因A379V和T403C位点多态性。用酶联免疫吸附法测定两组血脂及血浆Lp-PLA2水平,并进行组间比较。结果:与健康对照组比较,CHD组年龄、男性比例、血浆hs-cTnI、hsCRP、TC、LDL-C、Lp(a)、WBC、单核细胞数及Lp-PLA2水平[(119.98±49.41)ng/ml比(248.59±76.51)ng/ml]显著升高,HDL-C水平显著降低(P均<0.01)。两组Lp-PLA2基因A379V、T403C位点均检测到CC,CT,TT基因型及C,T等位基因。与健康对照组比较,CHD组Lp-PLA2基因T403C位点CC基因型(1.7%比9.3%)及C等位基因频率(13.7%比20.3%)均显著升高,P均<0.05;两组A379V基因位点的所有基因型及等位基因均无显著差异。结论:血浆Lp-PLA2水平可能与CHD发病风险有关。Lp-PLA2基因T403C位点多态性与冠心病遗传易感性之间具有一定关系。Objective： To study the relationship among lipoprotein-associated phospholipase A2 （Lp-PLA2） gene A379V and T403V locus polymorphisms and genetic susceptibility of coronary heart disease （CHD）. Methods： Lp-PLA2 gene A379V and T403V locus polymorphisms of 160 coronary angiography confirmed CHD patients （CHD group） and 117 healthy subjects （healthy control group） were measured using gene sequencing technique. ELISA was used to measure bloodlipids and plasma Lp-PLA2 level in two groups, and they were compared between two groups. Results： Compared with healthy control group, there were significant rise in age, male proportion, plasma levels of hs-cTnI, hsCRP, TC, LDL-C, Lp （a）, WBC, mononuclear cells （MNCs） and Lp-PLA2 [（119.98 ± 49. 41） ng/ml vs. （248. 59 ± 76. 51） ng/ml], and significant reduction in HDL-C level in CHD group （P〈0. 01 all）. The CC, CT, TT genotype and C, T allele were de- tected all in A379V and T403C locus of two groups. Compared with healthy control group, there were significant rise in frequencies of CC genotype （1.7% vs. 9. 3%） and C allele （13.7% vs. 20.3%） of Lp-PLA2 gene T403C locus in CHD group, P〈0. 05 both. All genotypes and alleles of A379V locus possessed no significant difference between CHD and healthy control group. Conclusion.. Plasma Lp-PLA2 level may be related to CHD risk. Lp-PLA2 gene T403C locus poly- morphism possesses certain relationship with genetic susceptibility of CHD.

关 键 词：磷脂酶类 基因多态性 单核苷酸 冠心病 

分 类 号：R541.409[医药卫生—心血管疾病]