纤维素酶高产细菌的筛选、鉴定及酶学特性分析  

Screening and Identification of a Cellulase Producing Bacterium and Its Cellulase Characterization

作　　者：李碧婵[1,2,3] 陈金燕[3] 苏雅娜[3] 张敏[1,2,3] 

机构地区：[1]福建省生态产业绿色技术重点实验室(武夷学院),福建武夷山354300 [2]福建省高校绿色化工技术重点实验室,福建武夷山354300 [3]武夷学院生态与资源工程学院,福建武夷山354300

出　　处：《湖北农业科学》2016年第17期4572-4576,共5页Hubei Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(201310397007)

摘　　要：从武夷山森林腐烂枯叶及附近土壤中筛选分离得到1株产纤维素酶活力较高的菌株CS-7。通过形态特征观察和16S rDNA序列分析,将其鉴定为蜡状芽胞杆菌（Bacillus cereus）。对菌株CS-7所产纤维素酶的相关酶学特性进行研究,结果表明,该菌株所产纤维素酶CMC酶活为7.91 U/mL,FPA酶活为2.40 U/mL;酶的最适反应温度和最适反应pH分别为60℃和6;在40-50℃温度范围内热稳定性较好;在pH 5.0-7.0范围内pH稳定性较好。研究结果表明,CS-7菌株是能产生耐高温中性纤维素酶的菌株,具有进一步研究开发利用的潜力。A cellulase producing strain CS-7 was isolated from soil samples collected from forest decayed leaf and soil around Wuyishan. Based on morphological and physiological characterization, CS-7 was characterized as Bacillus cereus. The enzymatic properties of cellulase from CS-7 were studied. The results showed that the CMCase activity of enzyme produced by CS-7 was 7.91 U / mL. The FPase activity was 2.40 U / mL. The optimal conditions for the enzymatic reaction were 60 ℃ and6.0. The enzyme preparations of CS-7 strain were stable at 40-50 ℃ and pH 5.0-7.0. These results indicated that microbial strain CS-7 could produce heat-resistant neutral cellulase which has great potential for further exploration and utilization.

关 键 词：纤维素酶 筛选 CMC酶活 滤纸酶活 酶学特性 

分 类 号：Q936[生物学—微生物学]