超微粉碎对莲子心活性成分提取率及抗氧化能力的影响  

Effects of Ultrafine Grinding on the Extraction Rate and Antioxidant Properties of the Active Ingredients of Lotus Plumule

作　　者：陈铭[1] 薛琪[1] 沈婷[1] 王新风[1] 强倩[1] 罗海青[1] 朱翠玲[1] 吴海峰[2] 吴磊[3] 李鹏霞[4] 胡卫成[1] CHEN Ming XUE Qi SHEN Ting WANG Xin-feng QIANG Qian LUO Hai-qing ZHU Cui-ling WU Hai-feng WU Lei LI Peng-xia HU Wei-eheng(Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huaian 223300, China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute ＆Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China College of Materials Science and Chemical Engineering, Tianjin University of Science and Technology, Tianjin 300457, China Institute of Agro-food Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

机构地区：[1]淮阴师范学院江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安223300 [2]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,北京100193 [3]天津科技大学材料科学与化学工程学院,天津300457 [4]江苏省农业科学院农产品加工研究所,江苏南京210014

出　　处：《现代食品科技》2016年第11期228-234,共7页Modern Food Science and Technology

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015A018);江苏省高校自然科学基金(14KJB550002);江苏省自然科学基金(BK20150414);国家科技支撑计划项目(2015BAD29B00)

摘　　要：为评价超微粉碎对莲子心粒径、微观结构、总黄酮和总酚提取率及抗氧化能力的影响,分别采用亚硝酸钠-硝酸铝法和福林法测定莲子心中总黄酮和总酚的含量,采用还原能力、DPPH自由基清除、ABTS+自由基清除能力、总氧自由基吸收能力以及细胞内抗氧化活性系统评价其抗氧化能力。结果表明:莲子心超微粉的中位粒径减小,细胞破壁率增加,总黄酮提取率提高了21.82%,总酚提取率提高了24.09%。抗氧化能力评价中,超微粉碎能明显提高莲子心的还原能力、DPPH和ABTS+自由基清除能力、总氧自由基吸收能力以及细胞内抗氧化活性。超微粉碎技术可以改善莲子心粉体的理化性质,提高莲子心抗氧化活性物质(总酚和总黄酮)的溶出率,同时超微粉碎处理显著提高抗氧化能力,可作为以莲子心为原料开发功能性食品的一种前处理手段。To evaluate the effect of ultrafine grinding on the particle size, microstructure, extraction rates of total phenolics and flavonoids, and antioxidant capacity of lotus plumule, the total phenolic and flavonoid contents were measured using Na NO2-Al（NO3）3 and Folin-phenol reagent methods, respectively. Antioxidant activity was evaluated by 2,2′-diphenyl-1-picrylhydrazyl（DPPH） free radical-scavenging activity, reducing power assay, 2,2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS） scavenging activity, oxygen radical absorbance capacity（ORAC）, and cellular antioxidant activity（CAA） assay. The results showed that for the samples treated by ultrafine grinding, the median particle size reduced, the cell wall-breaking rate increased, and the extraction rates of total flavonoids and phenolics increased by 21.82% and 24.09%, respectively. In terms of antioxidant capacity, ultrafine grinding treatment significantly enhanced DPPH free radical-scavenging activity, reducing power, ABTS scavenging activity, ORAC, and CAA. In conclusion, ultrafine grinding could improve the physicochemical properties of lotus plumule powder and increase the dissolution rates of the active ingredients（total phenolics and total flavonoids） in lotus plumule. Meanwhile, ultrafine grinding significantly increased antioxidant capacity and could serve as a pretreatment method in the development of functional food products with lotus plumule as the raw material.

关 键 词：超微粉碎 莲子心 自由基 抗氧化 

分 类 号：R284[医药卫生—中药学] TQ914[医药卫生—中医学]