DQB1＊060101 may contribute to susceptibility to immunoglobulin A nephropathy in southern Han Chinese  

作　　者：Wei Wang Ming Li Li Wang Xueqing Yu 

机构地区：[1]Department of Nephrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China [2]Key Laboratory of Nephrology, Ministry of Health and Guangdong Province, Guangzhou 510080, China [3]Department of Nephrology, Siehuan Provincial People's Hospital, Chengdu 610072, China

出　　处：《Frontiers of Medicine》2016年第4期507-516,共10页医学前沿（英文版）

基　　金：This work was funded by the Guangdong Department of Science and Technology Translational Medicine Center grant （No. 2011 A080300002）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （No. 20130171130008）, the Science and Technology Planning Project of Guangdong Province, China （Nos. 2013B051000019 and 2014B020212020）, Natural Science Foundation of Guangdong Province, China （No. 2014A030313136）, and National Natural Science Foundation of China （No. 81570599）. We thank the staff of The First Affiliated Hospital of Sun Yat-sen University for help with sample collection, DNA extraction, and clinical data collection.

摘　　要：Immunoglobulin A nephropathy （IgAN） is a common form of chronic glomerulonephritis with unknown pathogenesis. Accumulating evidences have shown the ethnic-specific association between certain human leukocyte antigen （HLA） alleles and IgAN susceptibility. This study was designed to explore the relationship between HLA-DQB1 alleles and disease susceptibility and clinical manifestations of patients with IgAN in southern Han Chinese. A PCR sequence-based typing technique was used to detect HLA-DQB1 alleles in 217 IgAN patients and 229 healthy subjects. Clinical data were collected from each patient at the time of renal biopsy. Twenty HLA-DQB1 alleles were detected in IgAN patients and healthy subjects. High frequency of HLA- DQB1＊060101 and low frequency of HLA-DQB1＊030101 were observed in IgAN patients compared with healthy controls. Further stratification analysis revealed that the frequency of DQB1＊060101 was significantly higher in patients with urine protein ≥1.0 g/24 h than in patients with urine protein 〈 1.0 g/24 h. In combination with our previous DRB1 results, we also analyzed the association of DRB1-DQB1 haplotypes with IgAN. We found that the frequency of haplotype DRBI＊090102-DQB1＊060101 was significantly higher [odds ratio （OR） = 4.409, Pc = 0.016], whereas that of HLA-DRBI＊070101-DQB1＊020101 was significantly lower （OR= 0.194, Pc = 0.016） compared with healthy controls. Our study indicated that HLA-DQB ＊060101 alleles may be a potential predictor of high-risk IgAN susceptibility in Chinese Han population.Immunoglobulin A nephropathy （IgAN） is a common form of chronic glomerulonephritis with unknown pathogenesis. Accumulating evidences have shown the ethnic-specific association between certain human leukocyte antigen （HLA） alleles and IgAN susceptibility. This study was designed to explore the relationship between HLA-DQB1 alleles and disease susceptibility and clinical manifestations of patients with IgAN in southern Han Chinese. A PCR sequence-based typing technique was used to detect HLA-DQB1 alleles in 217 IgAN patients and 229 healthy subjects. Clinical data were collected from each patient at the time of renal biopsy. Twenty HLA-DQB1 alleles were detected in IgAN patients and healthy subjects. High frequency of HLA- DQB1＊060101 and low frequency of HLA-DQB1＊030101 were observed in IgAN patients compared with healthy controls. Further stratification analysis revealed that the frequency of DQB1＊060101 was significantly higher in patients with urine protein ≥1.0 g/24 h than in patients with urine protein 〈 1.0 g/24 h. In combination with our previous DRB1 results, we also analyzed the association of DRB1-DQB1 haplotypes with IgAN. We found that the frequency of haplotype DRBI＊090102-DQB1＊060101 was significantly higher [odds ratio （OR） = 4.409, Pc = 0.016], whereas that of HLA-DRBI＊070101-DQB1＊020101 was significantly lower （OR= 0.194, Pc = 0.016） compared with healthy controls. Our study indicated that HLA-DQB ＊060101 alleles may be a potential predictor of high-risk IgAN susceptibility in Chinese Han population.

关 键 词：DQB 1 human leukocyte antigen （HLA） IgA nephropathy HAPLOTYPE association study 

分 类 号：R[医药卫生]