酶法提取牛骨胶原蛋白研究  

Study on the Extraction for Collagen from Bovine Bone by Enzymolysis

作　　者：陈静怡[1] 肖厚荣[1] 杨红[1] 张靖华 张佩佩[1] 朱许燕 

机构地区：[1]合肥学院生物与环境工程系,安徽合肥230601 [2]安徽华伊美科技企业集团,安徽合肥230088

出　　处：《蚌埠学院学报》2016年第6期31-35,共5页Journal of Bengbu University

基　　金：安徽省科技攻关项目(1301032168);安徽省大学生创新创业项目(201511059320)

摘　　要：以牛骨为原料,采用酶解法提取胶原蛋白,对影响胃蛋白酶酶解过程的诸多因素进行研究;在单因素实验的基础上进行正交优化,得到酶解法提取胶原蛋白的最佳工艺参数。结果表明:最佳酶解条件为脱脂时间6 h,脱钙时间6 h,加酶量0.50%,p H 1.8,酶解温度38℃,酶解时间8 h。在该条件下,牛骨胶原蛋白提取率最高,可以达到25.38%。The collagen of bovine bone has been extracted by using pepsin,and the effect of the main process parameters for the hydrolysis of bovine bone was investigated. On the basis of single factor test,orthogonal design was used to optimize the enzymolysis technology. The results showed that the optimal hydrolysis conditions for extracting collagen from bovine bone included degreasing time is 6 h,decalcifying time is 6 h,enzyme amount is 0. 50%,p H 1. 8,hydrolysis is at 38 ℃,enzymatic hydrolysis time is 8 h,at which the yield of collagen can reach the highest 25. 38% 。

关 键 词：牛骨 胶原蛋白 酶解法 正交设计 

分 类 号：TQ936[化学工程]