甘露聚糖酶微水固相法改性皂荚多糖胶  

β-Mannanase Modified Gleditsia sinensis Gum Using Micro-water-solid-phase Methods

作　　者：刘彦涛[1] 许正龙[1] 林雪娇[1] 孙达锋[2] 张卫明[2] 蒋建新[1] Liu Yantao Xu Zhenglong Lin Xuejiao Sun Dafeng Zhang Weiming Jiang Jianxin(College of Material Science and Technology, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, China)

机构地区：[1]北京林业大学材料科学与技术学院,林业生物质材料与能源教育部工程研究中心,北京100083 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2016年第6期12-16,共5页Chinese Wild Plant Resources

基　　金：国家重点研发计划课题(2016YFD0600803);国家自然科学基金项目(31270624)

摘　　要：作为功能性天然高分子,皂荚半乳甘露聚糖胶资源丰富。基于多糖胶吸水润胀特点提出的微水固相酶法改性皂荚多糖胶,在微水吸胀过程同时渗入酶进行改性反应,微水吸胀后的胚乳片经压片处理后呈蓬松多孔雪花片状,反应比表面积加大。β-甘露聚糖酶降解皂荚多糖胶,加酶量在3000~4000 U/（g胚乳）范围内,加水量为0.9倍胚乳片质量,在40 h反应时间后可得到最大低聚糖含量,为18.17%。微水固相法酶解半乳甘露聚糖可节约酶用量成本且易操作,避免了多糖胶溶液的高粘度性对酶解带来的不便,对多糖胶酶法改性的工业化应用有重要意义。Galactomannan gums are functional natural polymers, which are rich in the seeds of Gleditsia sinensis. Modification of galactomannan in micro - water - solid - phase is a innovative method proposed based on the swelling characteristics of the polysaccharide gums. While swelling in small amount of wa- ter, the enzymes were absorbed for the modification reaction, and imbibed endosperms were fluffy with snow flake after the pressure treatment. Modification of galactomannan using β - mannanase in micro - water- solid- phase obtained a maximum oligosaccharide content of 18.17 % , with the amount of en- zyme in 3000 -4000 U/（ g endosperm） , water： endosperm =0.9 and reaction for 40 h. Enzymatic mod- ification of galactomannan in micro - water - solid - phase saves the cost of enzyme. It is easily operated and successfully avoids the inconvenience of high viscosity of gum solution. This new method is suitable for the practical production and has a significant impact on the industrial application of polysaccharide gum modification.

关 键 词：皂荚 半乳甘露聚糖胶 微水固相 酶法改性 

分 类 号：Q539[生物学—生物化学]