分散液液微萃取-高效液相色谱法联用检测豆浆中双酚A的质量浓度  

Determination of bisphenol A in soybean milk by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography

作　　者：查一鸣 王新秀[1] 范永[1] 王田霖[1] 罗立强[1] ZHA Yiming WANG Xinxiu FAN Yong WANG Tianlin LUO Liqiang(College of Sciences, Shanghai University, Shanghai 200444, Chin)

机构地区：[1]上海大学理学院,上海200444

出　　处：《上海大学学报（自然科学版）》2016年第6期746-752,共7页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金资助项目(61571278)

摘　　要：建立一种采用分散液液微萃取-高效液相色谱法(dispersive liquid-liquid microextraction coupled with high performance liquid chromatography,DLLME-HPLC)联用对豆浆中的双酚A进行萃取和检测的新方法,并考察不同因素对萃取回收率和富集倍数的影响.实验结果表明,在最优化的萃取条件下,在1～100μg/L质量浓度范围内,双酚A的质量浓度与色谱峰面积呈良好的线性关系,检出限为0.3μg/L,加标回收率在91.6%～97.5%之间,相对标准偏差小于6%.这说明该方法的准确度和重现性较好,具有可行性,适用于对市售豆浆饮品中双酚A质量含量的测定.A novel method was developed for determination of bisphenol A in soybean milk using dispersive liquid-liquid microextraction coupled with high performance liquid chromatography （DLLME-HPLC）. Under the optimal conditions, the peak area had a linear relation with the concentration of bisphenol A over the range of 1-100 μg/L. The detection limit was 0.3 μg/L and recovery was among 91.6%-97.5%. The method had good accuracy and reproducibility, and was appropriate for determination of bisphenol A in soybean milk.

关 键 词：双酚A 分散液液微萃取 高效液相色谱法 豆浆 

分 类 号：TS207[轻工技术与工程—食品科学]