高速逆流色谱法分离纯化杜仲叶中绿原酸  

Isolation and purification of chlorogenic acid from Eucommia ulmoides Oliver. leaves by high-speed counter-current chromatography

作　　者：张敏[1] 李湘洲[1] 魏新莉[1] 王芳[1] 

机构地区：[1]中南林业科技大学材料科学与工程学院,长沙410004

出　　处：《中国食品添加剂》2016年第11期170-173,共4页China Food Additives

基　　金："十二五"科技支撑计划项目(2012BAD21B05);中南林业科技大学高层次人才引进项目(104-0268)

摘　　要：目的:建立高速逆流色谱(HSCCC)法从杜仲叶中分离纯化绿原酸。方法:将杜仲叶用50%乙醇提取,乙酸乙酯萃取,减压浓缩得粗提物,绿原酸含量为16.15%,再进行HSCCC分离纯化。用HPLC考察了绿原酸在不同溶剂体系中的分配情况,选择正丁醇-乙酸乙酯-水(3∶1∶4)为HSCCC为溶剂体系,上相为固定相,下相为流动相,主机转速850r/min,流速2m L/min,检测波长327nm。结果:从100mg杜仲粗提物中分离到8.27mg绿原酸,经HPLC分析绿原酸质量分数为86.20%,紫外、红外光谱扫描与对照品特征峰一致。结论 HSCCC法可用于杜仲叶中高纯度的绿原酸快速分离纯化。Object ： To isolate and purify of chlorogenic acid from Eucommia ulmoides Oliver.leaves crude extrac by high speed counter-current chromatography （HSCCC） . Methods ： The Eucommia ulmoides Oliver.leaves was extracted by 50% alcohol and then ethyl acetate. The crude extract was concentrated under reduced pressure and the content of chlorogenic acid was 16.15%, and further purified by HSCCC. HPLC was used to determine the partition coefficient of chlorogenic acid in different solvent system. N-Butyl alcohol-ethyl acetate-water （3 ： 1 ： 4, v/v/v） was chosen as two phase solvent system of HSCCC, the upper phase was used as the stationary phase , while the lower phase was used as the mobile phase with a flow rate of 2.0mL/min. The apparatus rotate was set at 850r/min, and detected at 327nm. Result Total of 8.23mg chlorogenic acid with high purity （86.3% analyzed by high performance liquid chromatography） was obtained from 100mg crude sample of Eucommia ulmoides Oliver. by HSCCC. The structure was identified by UV and IR. Conclusion ： HSCCC is a powerful technique for rapid isolation and purification of chlorogenic acid from Eucommia ulmoides Oliver. leaves.

关 键 词：高速逆流色谱 分离纯化 杜仲叶 绿原酸 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]