超高效液相色谱-串联四极杆-线性加速离子阱复合质谱法检测加工肉制品中6种合成着色剂  

Determination of 6 synthetic colorants in processed meat by ultra performance liquid chromatography-tandem quadrupole-linear accelerated ion trap mass spectrometry

作　　者：刘瑜[1] 房建美 吴渺渺 钟钰[1] 于丽[1] 李晓东[1] 赵颖[1] 金雁[1] 黄滢滢[1] 

机构地区：[1]沈阳出入境检验检疫局,沈阳110016

出　　处：《中国食品添加剂》2016年第11期204-207,共4页China Food Additives

基　　金：辽宁出入境检验检疫局科技计划项目(LK18-2015)

摘　　要：建立了加工肉制品中6种合成着色剂检测的超高效液相色谱-串联质谱分析方法。样品经甲醇-5%氨水（3∶7）混合溶液提取,正己烷除脂,经Oasis WAX固相萃取小柱净化,6种着色剂经Waters Acquity UPLC BEH C18色谱柱分离,以甲醇和0.02 mol/L乙酸铵水溶液为流动相进行梯度洗脱,电喷雾负离子（ESI-）模式电离。结果表明：6种色素在0.05~5.0μg/m L范围内呈良好的线性关系,相关系数（r）均大于0.991;酱牛肉样品中3个浓度添加水平平均回收率为83.5%~96.8%;相对标准偏差为3.84%~9.26%。该方法应用Qtrap特有的MRM-IDA-EPI扫描模式,可实现化合物的进一步确证。An ultra performance liquid chromatography-tandem mass spectrometry （UPLC MS/MS） method was established for the determination of 6 Synthetic colorants in processed meat. Samples were extracted with methanol-5% ammonia （30 ： 70, v/v） solution. Extraction liquid was purified by Hexane removal and Oasis HLB SPE column. The separation of 6 Synthetic colorants was performed on a Waters Acquity UPLC system with a BEH C18 column. The mobile phases were methanol and 0.02 mol/L ammonium acetate in gradient elution. The electrospray was operated in the negative ionization mode. The calibration curves showed good linearity within the concentrations of 0.05 - 5.0 mg/mL with the correlation coefficients r 〉 0.991. The recoveries of 6 Synthetic colorants were 83.5% - 96.8% at the spiked levels of 0.1, 0.2, 1.0 mg/kg. The relative standard deviations coefficients of variation were both less than 3.84% - 9.26%. This method can be used to further confirm the compounds by using the Qtrap specific MRM-IDA-EPI scanning mode.

关 键 词：超高效液相色谱-串联四极杆-线性加速离子阱复合质谱 合成着色剂 加工肉制品 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]