组氨酸标签蛋白纯化介质的合成及应用  

Synthesis of Purification Media of the Histidine Tag Protein and Its Application

作　　者：华伟伟[1] 徐维元[1] 黄建颖[1] 杨利军[1] 

机构地区：[1]浙江工商大学食品与生物工程学院浙江省果蔬保鲜与加工技术研究重点实验室,杭州310018

出　　处：《中国食品学报》2016年第10期80-86,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省重大科技专项(2011C12031);国家自然科学青年基金项目(21102129)

摘　　要：以离子液体为萃取溶剂,将氮杂大环功能性配体与疏水性离子液体链接构建亲和性离子液体,通过氮杂大环螯合金属离子构成复合物;利用螯合的金属离子(Cu2+、Ni2+、Zn2+)对组氨酸标签特异的亲和性,对组氨酸标签蛋白进行特异性亲和纯化,结果表明:AIL螯合金属离子后才会对FITC-(His)6-OH产生亲和性。Cu-AIL亲和性最强,对于His-tag EGFP的选择性最差;Ni-AIL受限于其低的金属离子结合量,获得的目的蛋白最少;Zn-AIL能有效亲和His-tag EGFP,纯化效率相对最佳。结合以上不同亲和介质的特性,开发出利用Zn-AIL与Cu-AIL的二次纯化体系,获得纯度约90%的His-tag EGFP。Synthesize affinity ionic liquid（AIL） through linking polyazamacrolyle with hydrophobic ionic liquid based on the designability using ionic liquids as extraction solvents. Affinity ionic liquid（AIL） is used for the purification of histidine tag protein relying on the affinity resulted from the complexes chelated by metal ion（Cu2 ＋、Ni2 ＋、Zn2 ＋） and polyazamacrocyle. The results showed that only when AIL chelated by metal ion, can it attach to the FITC-（His）6-OH.Cu-AIL exhibited the best affinity ability for his-tag EGFP but selective ability was the worst one; Ni-AIL was limited by its low amount of metal ion combination, so the purpose protein is the least; Zin-AIL could attach the his-tag EGFP efficiently, possessing the best purification efficiency. And combined with the above different affinity media features, we developed the secondary purification system with Zn-AIL and Cu-AIL, obtained the purity of about 90% of His-tag EGFP.

关 键 词：亲和性离子液体 螯合 组氨酸标签 蛋白质纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]