高效液相色谱法同时测定面制品中顺丁烯二酸和富马酸的含量  

Simultaneous determination of maleic acid and fumaric acid in flour products by high performance liquid chromatography method

作　　者：雍宗锋 曹慧[1] 周新亚[1] 

机构地区：[1]宿迁市疾病预防控制中心,江苏宿迁223800

出　　处：《中国食品卫生杂志》2016年第6期769-772,共4页Chinese Journal of Food Hygiene

基　　金：江苏省预防医学研究课题(Y2015048)

摘　　要：目的建立用高效液相色谱法同时测定面制品中的顺丁烯二酸和富马酸含量。方法样品经50 ml 10%甲醇-水萃取,离心后,水层过0.45μm滤膜,经Waters Atlantis T3色谱柱（250 mm×4.6 mm,5μm）分离,以0.1%磷酸-甲醇（95∶5,V/V）作流动相,紫外检测器检测,波长210 nm。结果在优化的试验条件下,顺丁烯二酸和富马酸的浓度在0.1~25μg/ml范围内与峰面积呈良好的线性关系,回归方程分别为y（顺丁烯二酸）=147008x-1316.8（r=0.999 5,n=6）和y（富马酸）=176689x-670.1（r=0.999 6,n=6）,方法检出限均为2.0 mg/kg,回收率为86.88%~93.02%,相对标准偏差为0.67%~1.08%。结论该方法简便、准确,可适用于面制品中顺丁烯二酸和富马酸的同时测定。Objective To develop a high performance liquid chromatography method for the determination of maleic acid and fumaric acid in flour products. Methods The flour product samples were extracted with 50 ml of 10% methanol and water,then centrifuged and filtered with 0. 45 μm membrane. Waters Atlantis T3 chromatographic column was used to separate the sample with 0. 1% phosphoric acid and methyl alcohol（ 95 ∶ 5,V / V） as the mobile phase. The samples were detected by UV-detector,the column temperature was 30 ℃ and the wavelength was 210 nm. Results Under the optimized experimental conditions,the calibration curve showed good linearity in the range of 0. 1-25 μg / ml. The regression equation is y = 147008x- 1316. 8（ r = 0. 999 5,n = 6） for maleic acid,y = 176689x- 670. 1（ r = 0. 999 6,n = 6） for fumaric acid. The detection limit was 2. 0 mg / kg with the recovery rate in the range of 86. 88%-93. 02%,and the relative standard deviation of method was 0. 67%-1. 08%. Conclusion The method was simple,accurate,and could be applied to the determination of maleic acid and fumaric acid in flour products.

关 键 词：高效液相色谱法 面制品 顺丁烯二酸 富马酸 食品污染物 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.72[医药卫生—公共卫生与预防医学]