小金海棠多酚对高脂饮食小鼠的降脂作用及机制  

Lipid-lowering Function and the Mechanism of Malus xiaojinensis Polyphenols on Mice(Mus musculus) with High-fat Diet

作　　者：韦月平[1] 王鹏[1] 

机构地区：[1]辽东学院农学院,丹东118003

出　　处：《农业生物技术学报》2017年第1期67-73,共7页Journal of Agricultural Biotechnology

基　　金：辽东学院青年基金

摘　　要：本研究旨在探讨小金海棠多酚(Malus xiaojinensis polyphenols,MXP)的降脂减肥作用及其机制。采用饲喂高脂饲料建立高脂小鼠(Mus musculus)模型,小金海棠多酚灌胃给药(50,100 mg/kg),连续7周。检测血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)和肝组织中TG与TC水平,并计算肝脾指数。同时检测肝组织中白细胞介素-6(interleukin-6,IL-6)与肿瘤坏死因子(tumor necrosis factor-α,TNF-α)转录情况并观察肝组织形态。最后检测糖脂代谢途径相关蛋白胰岛素受体底物(insulin receptor substrate,IRS)和蛋白激酶B(Akt)的表达状况。结果显示,MXP可以显著抑制小鼠体重快速增长并降低血清和肝组织中TG、TC及LDL-C的水平,同时提高HDL-C的水平。此外,MXP能显著抑制包括IL-6及TNF-α在内的炎性细胞因子的生成及肝细胞损伤。更为重要的是MXP可以显著提高IRS-1和Akt的表达。因此,小金海棠多酚的降脂功能很可能与其能提高葡萄糖的利用率和抑制炎性细胞因子活性有关,具有开发成为降脂减肥功能食品及保健品的潜力。本研究为科学合理开发小金海棠(Malus xiaojinensis)提供理论基础和依据。The study was to investigate the lipid-lowering and anti-obesity function and mechanism of Malus xiaojinensis polyphenols（MXP）. Hyperlipemia mice（Mus musculus） model was established by feeding highfat diet and MXP gavage administration（50 and 100 mg/kg） for 7 weeks. The levels of triglyceride（TG）, total cholesterol（TC）, low density lipoprotein-cholesterol（LDL-C） and high density lipoprotein-cholesterol（HDLC） in serum, the TG and TC in liver, the liver and spleen index were measured. At the same time, the transcription of interleukin-6（IL-6）, tumor necrosis factor-α（TNF-α） and the morphology of liver tissue were detected. Finally, the expression of related proteins（IRS, Akt） in the pathway of glucose and lipid metabolism was detected. The results showed that MXP could obviously resist mice body weight quick increase, MXP could significantly reduce the levels of TG, TC and LDL-C in the serum, TG and TC in liver, and increased the level of HDL-C in mice. MXP also could reduce the inflammatory cytokines including IL-6 and TNF-αand liver injury. Furthermore, MXP could improve the expression of IR and IRS significantly. The MXP seemed to improve the utilization rate of glucose and reduce the levels of inflammatory cytokines in liver, it has the potential to develop into a functional food and health care product. This study provides theoretical basis for scientific and rational development of M. xiaojinensis.

关 键 词：小金海棠 多酚 降脂 机制 

分 类 号：S184[农业科学—农业基础科学] TS201.4[轻工技术与工程—食品科学]